Ultrasensitive CRISPR-based diagnostic for field-applicable detection of Plasmodium species in symptomatic and asymptomatic malaria

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 117; no. 41; pp. 25722 - 25731
• Main Authors: Lee, Rose A., De Puig, Helena, Nguyen, Peter Q., Angenent-Mari, Nicolaas M., Donghia, Nina M., McGee, James P., Dvorin, Jeffrey D., Klapperich, Catherine M., Pollock, Nira R., Collins, James J.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 13-10-2020
• Series: From the Cover
• Subjects: Assaying; Asymptomatic; Biological Sciences; Blood; Carrier State - diagnosis; Carrier State - parasitology; Clustered Regularly Interspaced Short Palindromic Repeats; CRISPR; Density; Diagnostic systems; Diagnostic Techniques and Procedures; Erythrocytes; Fluorescence; Genetic Techniques; Humans; Infections; Malaria; Malaria - diagnosis; Malaria - parasitology; Nucleic acids; Parasites; Plasmodium; Plasmodium - classification; Plasmodium - genetics; Plasmodium - isolation & purification; Plasmodium - physiology; Plasmodium falciparum; Sample preparation; Sensitivity; Species; Vector-borne diseases; CRISPR-Dx; diagnostics; SHERLOCK; malaria
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.2010196117

Asymptomatic carriers of Plasmodium parasites hamper malaria control and eradication. Achieving malaria eradication requires ultrasensitive diagnostics for low parasite density infections (<100 parasites per microliter blood) that work in resource-limited settings (RLS). Sensitive point-of-care diagnostics are also lacking for nonfalciparum malaria, which is characterized by lower density infections and may require additional therapy for radical cure. Molecular methods, such as PCR, have high sensitivity and specificity, but remain high-complexity technologies impractical for RLS. Here we describe a CRISPR-based diagnostic for ultrasensitive detection and differentiation of Plasmodium falciparum, Plasmodium vivax, Plasmodium ovale, and Plasmodium malariae, using the nucleic acid detection platform SHERLOCK (specific high-sensitivity enzymatic reporter unlocking). We present a streamlined, field-applicable, diagnostic comprised of a 10-min SHERLOCK parasite rapid extraction protocol, followed by SHERLOCK for 60 min for Plasmodium species-specific detection via fluorescent or lateral flow strip readout. We optimized one-pot, lyophilized, isothermal assays with a simplified sample preparation method independent of nucleic acid extraction, and showed that these assays are capable of detection below two parasites per microliter blood, a limit of detection suggested by the World Health Organization. Our P. falciparum and P. vivax assays exhibited 100% sensitivity and specificity on clinical samples (5 P. falciparum and 10 P. vivax samples). This work establishes a field-applicable diagnostic for ultrasensitive detection of asymptomatic carriers as well as a rapid point-of-care clinical diagnostic for nonfalciparum malaria species and low parasite density P. falciparum infections.