Meiotic viral attenuation through an ancestral apoptotic pathway

The programmed release of apoptogenic proteins from mitochondria is a core event of apoptosis, although ancestral roles of this phenomenon are not known. In mammals, one such apoptogenic protein is Endonuclease G (EndoG), a conserved mitochondrial nuclease that fragments the DNA of dying cells. In t...

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Bibliographic Details
Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 116; no. 33; pp. 16454 - 16462
Main Authors:	Gao, Jie, Chau, Sabrina, Chowdhury, Fuad, Zhou, Tina, Hossain, Saif, McQuibban, G. Angus, Meneghini, Marc D.
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences 13-08-2019
Series:	From the Cover
Subjects:	Animals Apoptosis Apoptosis - genetics Attenuation Biological Sciences Deoxyribonucleic acid Derepression DNA Double-stranded RNA Endodeoxyribonucleases - genetics Endonuclease Endonucleases - genetics Exonucleases - genetics Homology Mammals Meiosis Meiosis - genetics Mitochondria Mitochondria - enzymology Mitochondria - genetics Nuclease PNAS Plus Progeny Proteins Saccharomyces cerevisiae Proteins - genetics Saccharomycetales - genetics Saccharomycetales - growth & development Saccharomycetales - virology Spores, Fungal - genetics Spores, Fungal - growth & development Sporulation Yeast Yeasts Nuc1 viruses gametogenesis mitochondria yeast
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Summary:	The programmed release of apoptogenic proteins from mitochondria is a core event of apoptosis, although ancestral roles of this phenomenon are not known. In mammals, one such apoptogenic protein is Endonuclease G (EndoG), a conserved mitochondrial nuclease that fragments the DNA of dying cells. In this work, we show that budding yeast executes meiotically programmed mitochondrial release of an EndoG homolog, Nuc1, during sporulation. In contrast to EndoG’s ostensible pro-death function during apoptosis, Nuc1 mitochondrial release is pro-survival, attenuating the cytosolic L-A and Killer double-stranded RNA mycoviruses and protecting meiotic progeny from the catastrophic consequences of their derepression. The protective viral attenuation role of this pathway illuminates a primordial role for mitochondrial release of EndoG, and perhaps of apoptosis itself.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by Reed B. Wickner, National Institutes of Health, Bethesda, MD, and approved June 14, 2019 (received for review January 16, 2019) Author contributions: J.G., S.C., F.C., T.Z., S.H., and M.D.M. designed research; J.G., S.C., F.C., T.Z., S.H., and M.D.M. performed research; G.A.M. contributed new reagents/analytic tools; J.G., S.C., F.C., T.Z., S.H., G.A.M., and M.D.M. analyzed data; and J.G. and M.D.M. wrote the paper.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.1900751116