Decolorization of salt-alkaline effluent with industrial reactive dyes by laccase-producing basidiomycetes strains

• Published in: Letters in applied microbiology Vol. 56; no. 4; pp. 283 - 290
• Main Authors: Moreira Neto, S. L., Mussatto, Solange I., Machado, K. M. G., Milagres, Adriane M. F.
• Format: Journal Article
• Language: English
• Published: Oxford Blackwell Publishing Inc 01-04-2013; Wiley; Blackwell; Oxford University Press
• Subjects: Basidiomycetes; Basidiomycota - enzymology; Basidiomycota - growth & development; Basidiomycota - metabolism; Biological and medical sciences; Coloring Agents - metabolism; Culture Media; Decolorization; Fundamental and applied biological sciences. Psychology; Hydrogen-Ion Concentration; Laccase; Laccase - metabolism; Microbiology; Nitrogen - metabolism; Oxidation-Reduction; Peroxidases; Reactive dyes; Salts; Science & Technology; Textiles; Waste Water; Discoloration; Dyes; Enzyme; Applied microbiology; decolorization; reactive dyes; Basidiomycota; Strain; Fungi; basidiomycetes; Salt; Peroxidases; Peroxidase; Oxidoreductases; Effluent; Laccase
• ISSN: 0266-8254; 1472-765X
• DOI: 10.1111/lam.12049

The discharge of highly coloured synthetic dye effluents into rivers and lakes is harmful to the water bodies, and therefore, intensive researches have been focussed on the decolorization of wastewater by biological, physical or chemical treatments. In the present study, 12 basidiomycetes strains from the genus Pleurotus, Trametes, Lentinus, Peniophora, Pycnoporus, Rigidoporus, Hygrocybe and Psilocybe were evaluated for decolorization of the reactive dyes Cibacron Brilliant Blue H-GR and Cibacron Red FN-2BL, both in solid and liquid media. Among the evaluated fungi, seven showed great ability to decolorize the synthetic textile effluent, both in vivo (74–77%) or in vitro (60–74%), and laccase was the main ligninolytic enzyme involved on dyes decolorization. Pleurotus ostreatus, Trametes villosa and Peniophora cinerea reduced near to 60% of the effluent colour after only 1 h of treatment. The decolorization results were still improved by establishing the nitrogen source and amount to be used during the fungal strains cultivation in synthetic medium previous their action on the textile effluent, with yeast extract being a better nitrogen source than ammonium tartarate. These results contribute for the development of an effective microbiological process for decolorization of dye effluents with reduced time of treatment.
The authors gratefully acknowledge CNPq (Brazil) for the financial support.