Association of insertion/deletion polymorphism of alpha-adrenoceptor gene in essential hypertension with or without type 2 diabetes mellitus in Malaysian subjects

• Published in: International journal of biological sciences Vol. 4; no. 6; pp. 362 - 367
• Main Authors: Vasudevan, R, Ismail, Patimah, Stanslas, Johnson, Shamsudin, Norashikin, Ali, Aisyah Binti
• Format: Journal Article
• Language: English
• Published: Australia Ivyspring International Publisher 01-01-2008
• Subjects: Adult; Aged; Case-Control Studies; Diabetes Mellitus, Type 2 - complications; Diabetes Mellitus, Type 2 - genetics; Female; Gene Frequency; Genotype; Humans; Hypertension - complications; Hypertension - genetics; INDEL Mutation; Malaysia; Male; Middle Aged; Receptors, Adrenergic, alpha-2 - genetics; Research Paper; Malaysia; Essential hypertension; Type 2 Diabetes Mellitus; Insertion/Deletion polymorphism; Alpha2B-Adrenoceptor
• ISSN: 1449-2288; 1449-2288
• DOI: 10.7150/ijbs.4.362

An insertion/deletion (I/D) polymorphism of Alpha2B-Adrenoceptor (ADRA2B) gene located on chromosome 2 has been studied extensively in related to cardiovascular diseases. The main aim of the present study was to examine the potential association of D allele frequency of I/D polymorphism of ADRA2B gene in Malaysian essential hypertensive subjects with or without type 2 diabetes mellitus (T2DM). This study includes 70 hypertensive subjects without T2DM, 65 hypertensive subjects with T2DM and 75 healthy volunteers as control subjects. Genotyping of I/D polymorphism was performed by conventional PCR method. There was significant difference found in age, body mass index, systolic/diastolic blood pressure and high density lipoprotein cholesterol level between the case and control subjects. DD genotypic frequency of I/D polymorphism was significantly higher in hypertensive subjects (42.84% vs. 29.33%; P-=0.029) and in hypertensive with T2DM subjects (46.15% vs. 29.33%; P=0.046) than control group. D allele frequency was higher in hypertensive group (67.41%) than control subjects (52.67%). However, no significant difference was found between the three genotypes of I/D polymorphism of ADRA2B gene and the clinical characteristics of the subjects. The result obtained in this study show D allele of ADRA2B gene was associated with essential hypertension with or without T2DM in Malaysian subjects.