Mathematical quantification of the induced stress resistance of microbial populations during non-isothermal stresses

• Published in: International journal of food microbiology Vol. 266; pp. 133 - 141
• Main Authors: Garre, Alberto, Huertas, Juan Pablo, González-Tejedor, Gerardo A., Fernández, Pablo S., Egea, Jose A., Palop, Alfredo, Esnoz, Arturo
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 02-02-2018; Elsevier BV
• Subjects: Acclimation; Acclimatization; Construction; Construction methods; Deactivation; E coli; Heat; Heat transfer; Heating rate; Inactivation; Induced stress resistance; Mathematical modelling; Mathematical models; Microbial inactivation; Microorganisms; Model testing; Parameter estimation; Stress; Temperature effects; Thermal resistance; Thermal stress; Microbial inactivation; Mathematical modelling; Induced stress resistance
• ISSN: 0168-1605; 1879-3460
• DOI: 10.1016/j.ijfoodmicro.2017.11.023

This contribution presents a mathematical model to describe non-isothermal microbial inactivation processes taking into account the acclimation of the microbial cell to thermal stress. The model extends the log-linear inactivation model including a variable and model parameters quantifying the induced thermal resistance.
The model has been tested on cells of Escherichia coli against two families of non-isothermal profiles with different constant heating rates. One of the families was composed of monophasic profiles, consisting of a non-isothermal heating stage from 35 to 70°C; the other family was composed of biphasic profiles, consisting of a non-isothermal heating stage followed by a holding period at constant temperature of 57.5°C. Lower heating rates resulted in a higher thermal resistance of the bacterial population. This was reflected in a higher D-value. The parameter estimation was performed in two steps. Firstly, the D and z-values were estimated from the isothermal experiments. Next, the parameters describing the acclimation were estimated using one of the biphasic profiles. This set of parameters was able to describe the remaining experimental data.
Finally, a methodology for the construction of diagrams illustrating the magnitude of the induced thermal resistance is presented. The methodology has been illustrated by building it for a biphasic temperature profile with a linear heating phase and a holding phase. This diagram provides a visualization of how the shape of the temperature profile (heating rate and holding temperature) affects the acclimation of the cell to the thermal stress. This diagram can be used for the design of inactivation treatments by industry taking into account the acclimation of the cell to the thermal stress.
•A mathematical model for non-isothermal microbial inactivation is developed.•The model considers the acclimation of the cell to the thermal stress.•The model extends the Bigelow inactivation model with terms quantifying the acclimation.•A methodology for creating a diagram illustrating the magnitude of the acclimation is presented.