Differentiation among isolates of prunus necrotic ringspot virus by transcript conformation polymorphism

• Published in: Journal of virological methods Vol. 74; no. 1; pp. 109 - 115
• Main Authors: Rosner, A, Maslenin, L, Spiegel, Sara
• Format: Journal Article
• Language: English
• Published: London Elsevier B.V 01-09-1998; Amsterdam Elsevier; New York, NY
• Subjects: Biological and medical sciences; Fundamental and applied biological sciences. Psychology; Ilarvirus - classification; Ilarvirus - genetics; Ilarvirus - isolation & purification; ILARVIRUSES; Microbiology; PCR; Phytopathology. Animal pests. Plant and forest protection; Plant viruses and viroids; Polymerase Chain Reaction; Polymorphism, Genetic; Polymorphism, Restriction Fragment Length; PRUNUS; Prunus necrotic ringspot virus; Strain differentiation; Systematics. Structure, properties and multiplication. Genetics; Techniques used in virology; TRANSCRIPTION; Transcription, Genetic; Virology; Strain differentiation; Transcription; Prunus necrotic ringspot virus; PCR; Ilarviruses; Plant pathogen; Method; Virus; Characterization; Polymerase chain reaction; Messenger RNA; Bromoviridae; Isolate; Single stranded RNA; Ilarvirus; Comparative study; Polymorphism
• ISSN: 0166-0934; 1879-0984
• DOI: 10.1016/S0166-0934(98)00076-7

A method based on differences in electrophoretic mobility of RNA transcripts made from polymerase chain reaction (PCR) products was used for differentiation among virus isolates. A T7 RNA polymerase promoter was attached to amplified prunus necrotic ringspot virus (PNRSV) sequences by PCR. The PCR products then served as a template for transcription. Single-stranded transcripts originated from different PNRSV isolates varied in electrophoretic mobility in polyacrylamide gels, presumably because of transcript conformation polymorphism (TCP). This procedure was applied for the differentiation of PNRSV isolates.