Effects of derivatives of hydroxypyruvaldehyde phenylosazone on bovine erythrocyte membrane. I. Influence on the osmotic fragility and morphology

• Published in: Chemical & pharmaceutical bulletin Vol. 30; no. 3; pp. 959 - 965
• Main Authors: Ikezava, H, Mizuno, M, Nakabayashi, T, Ogawa, H, Shindo, M, Matsunaga, I
• Format: Journal Article
• Language: English
• Published: Japan The Pharmaceutical Society of Japan 1982; Japan Science and Technology Agency
• Subjects: Aldehydes - chemical synthesis; animal physiology; Animals; Cattle; Erythrocyte Membrane - drug effects; Erythrocyte Membrane - ultrastructure; erythrocytes; Erythrocytes - drug effects; hydroxypyruvaldehyde phenylosazone; hypotonic shock; In Vitro Techniques; Osmotic Fragility - drug effects; osmotic stability; Pyruvaldehyde - analogs & derivatives; Pyruvaldehyde - chemical synthesis; Pyruvaldehyde - pharmacology
• ISSN: 0009-2363; 1347-5223
• DOI: 10.1248/cpb.30.959

Thirteen derivatives of hydroxypyruvaldehyde phenylosazone were tested for effects on bovine erythrocyte membrane. Six drugs, including hydroxypyruvaldehyde phenylosazone itself and the o-CH3, m-CH3, p-CH3, o-Cl and p-Cl derivatives, increased the stability of bovine erythrocyte membrane to hypotonic shock ; the other derivatives with highter molecular weights were essentially without effect. Observations by scanning electron microscopy indicated that non-substituted, m-CH3 and o-Cl hydroxypyruvaldehyde phenylosazones caused extrusion of the erythrocyte surface, whereas o-CH3, p-CH3 and p-Cl derivatives caused invagination of the erythrocyte membrane. The p-Cl derivative can be adsorbed onto, or incorporated into the bovine erythrocyte membrane.