In vitro antioxidant/prooxidant effects of combined use of flavonoids

• Published in: Natural product research Vol. 32; no. 12; pp. 1446 - 1450
• Main Authors: Eren-Guzelgun, B., Ince, E., Gurer-Orhan, H.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-06-2018; Taylor & Francis Ltd
• Subjects: Adult; Animal models; Animals; antioxidant; Antioxidants; Antioxidants - pharmacology; biphasic effect; combined effect; Daidzein; Drug Therapy, Combination; Erythrocytes; Erythrocytes - drug effects; Flavonoid; Flavonoids; Flavonoids - pharmacology; Fluorescent Dyes - chemistry; Fluorescent indicators; Genistein; Genistein - pharmacology; Hemolysis - drug effects; Humans; Hydrogen peroxide; Hydrogen Peroxide - pharmacology; Isoflavones - pharmacology; Lipid peroxidation; Lipid Peroxidation - drug effects; Male; Metabolites; Microsomes; Oxidants - pharmacology; Oxidation; Oxidation-Reduction; Peroxidation; Physiological effects; prooxidant; Quercetin; Quercetin - pharmacology; Rats, Wistar; Reactive Oxygen Species; antioxidant; combined effect; biphasic effect; prooxidant; Flavonoid
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2017.1346637

The present study was undertaken to investigate the individual and combined antioxidant or prooxidant effects of genistein, daidzein and quercetin in human erythrocytes and rat microsomes in vitro. Their reducing potential against oxidation of a redox sensitive fluorescent probe, their protective effect against H
2
O
2
-induced membrane lipid peroxidation and their inhibitory effect on AAPH-induced hemolysis were evaluated. Genistein and daidzein were prooxidant in erythrocytes but antioxidant in microsomes where their metabolites might have been formed which suggests the importance of metabolic capacity in in vitro models to predict the physiological situation. Quercetin showed antioxidant effects in all models and conditions. Prooxidant effect of 'genistein-daidzein mixture', at their concentrations reflecting the real life, was suppressed by addition of quercetin to the mixture. Our study shows that flavonoids can exert prooxidant effects depending on the conditions, but the mixture effect should be considered while assessing their effects and safety in humans.