Feasible Influence of G-CSF on Clinical Pregnancy Outcome in Oocyte Donation Cycles for Patients with Recurrent Implantation Failure

• Published in: Medicina (Kaunas, Lithuania) Vol. 60; no. 6; p. 966
• Main Authors: Kushniruk, Nataliya, Stastna, Anna, Fait, Tomas, Lenertova, Tereza
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 11-06-2024; MDPI
• Subjects: Adult; Age; Body mass index; Care and treatment; Chronic diseases; Cohort Studies; Donations; Embryo Implantation - drug effects; Embryo Transfer - methods; Embryos; endometrial receptivity; Endometrium; Failure; Female; Granulocyte Colony-Stimulating Factor - therapeutic use; Growth factors; Humans; implantation failure; In vitro fertilization; intrauterine lavage of G-CSF; Menstruation; Oocyte Donation - methods; oocyte donation cycle; Patient outcomes; Pregnancy; Pregnancy Outcome; Pregnancy Rate; Pregnant women; Womens health; Czech Republic; United States > US; implantation failure; intrauterine lavage of G-CSF; endometrial receptivity; oocyte donation cycle; pregnancy rate
• ISSN: 1648-9144; 1010-660X; 1648-9144
• DOI: 10.3390/medicina60060966

The aim of our single-center cohort study was the determination of the influence of the intrauterine lavage of granulocyte colony-stimulating growth factor (G-CSF) on clinical pregnancy rate in patients with a history of implantation failure older than 40 years.
The study was conducted in Ferticare Prague SE between May 2018 and June 2020. Overall, 115 patients were distributed into two arms, with 48 subjects in the experimental and 67 in the control arm. All women have had a previous history of unsuccessful history of infertility treatment with their own genetic material and at least one ineffective cycle with the donated oocytes. The experimental arm underwent the intrauterine lavage of 0.5 mL of pure G-CSF from 120 to 48 h prior to embryo transfer.
The clinical pregnancy rate was 63.3% in the experimental arm and 47.8% in the control arm (
= 0.097 for Pearsonߣs χ
, and
= 0.133 for Fisher's exact test). However, the mean endometrial thickness on the day of embryo transfer did not appear to be statistically different (
= 0.139). Only the difference in endometrium thickness growth was statistically significant (
= 0.023). The increase in pregnancy rate is still encouraging for the future, even if it is not significant.
Our study suggests the trend of increased pregnancy rate after the intrauterine G-CSF lavage in the interval of 120-48 h prior to embryo transfer.