Phase-assisted synthesis and DNA unpacking evaluation of biologically inspired metallo nanocomplexes using peptide as unique building block

• Published in: Journal of colloid and interface science Vol. 364; no. 1; pp. 140 - 147
• Main Authors: Raman, N., Sudharsan, S.
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier Inc 01-12-2011; Elsevier
• Subjects: Anti-Infective Agents - chemical synthesis; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Bacteria - cytology; Bacteria - drug effects; Bacteria - growth & development; Biological inspiration; Brust–Schiffrin; cell walls; Chemistry; Colloidal state and disperse state; DNA; DNA - chemistry; DNA unpacking; Exact sciences and technology; fungi; Fungi - cytology; Fungi - drug effects; Fungi - growth & development; General and physical chemistry; Membranes; microbial growth; Microbial Sensitivity Tests; Molecular Structure; mortality; Nanocomplex; nanomaterials; Nanostructures - chemistry; Organometallic Compounds - chemical synthesis; Organometallic Compounds - chemistry; Organometallic Compounds - pharmacology; Particle Size; Peptide; Peptides - chemistry; Structure-Activity Relationship; Surface Properties; Brust–Schiffrin; Biological inspiration; Peptide; DNA unpacking; Nanocomplex; Inspiration; Modification; Peptides; Growth; Brust-Schiffrin; Characterization; Propene; Synthesis; DNA; Membrane; Biomaterial; Recognition
• ISSN: 0021-9797; 1095-7103
• DOI: 10.1016/j.jcis.2011.08.034

Novel metallo nanocomplexes have been synthesized
via modified Brust–Schiffrin methodology using peptide as their building block. Biological inspiration of all the nanocomplexes is driven by their embedding peptide PrHA.
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► Novel metallo nanocomplexes synthesized
via modified Brust–Schiffrin approach. ► Among all the products, zinc and cobalt nanocomplexes unpack the DNA most efficiently. ► Biological inspirations of nanocomplexes are proved by targeting bacterial and fungal strains. ► Biological inspiration of the metallo nanocomplexes is driven by their size and the peptide PrHA.
The goal of nanomaterials’ surface modification using a biomaterial is to preserve the materials’ bulk properties while modifying only their surface to possess desired recognition and specificity. Here, we have developed a phase-assisted, modified Brust–Schiffrin methodological synthesis of metallo nanocomplexes anchored by a peptide, N,N′-(1,3-propylene)-
bis-hippuricamide. The spectral, thermal and morphological characterizations assure the formation of nanocomplexes. Therapeutic behavior of all the nanocomplexes has been well sighted by evaluating their DNA unpacking skills. In addition, we demonstrate their biological inspiration by targeting few bacterial and fungal strains. The
in vitro antimicrobial investigation reports that all the nanocomplexes disrupt microbial cell walls/membranes efficiently and inhibit the growth of microbes. These sorts of nanocomplexes synthesized in large quantities and at low cost, deliver versatile biomedical applications, and can be used to treat various diseases which may often cause high mortality.