Creating knockout and knockin rodents using engineered endonucleases via direct embryo injection

Genetically engineered rodents have been generated worldwide for biomedical research. Recently, gene targeting techniques have been developed by using engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly intersp...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 1239; p. 307
Main Authors:	Kaneko, Takehito, Mashimo, Tomoji
Format:	Journal Article
Language:	English
Published:	United States 01-01-2015
Subjects:	Animals Embryo Transfer - methods Embryo, Mammalian Endonucleases - genetics Endonucleases - metabolism Female Fertilization in Vitro - methods Gene Knock-In Techniques Gene Knockout Techniques Genetic Engineering - methods Genotyping Techniques Male Mice Microinjections - methods Rats RNA, Messenger - administration & dosage RNA, Messenger - genetics Rodentia
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Summary:	Genetically engineered rodents have been generated worldwide for biomedical research. Recently, gene targeting techniques have been developed by using engineered endonucleases such as zinc-finger nucleases (ZFN), transcription activator-like effector nucleases (TALEN) and clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. These endonucleases are useful for simple and rapid production of gene knockout/knockin animals without using embryonic stem (ES) cells. This chapter introduces the latest protocols for producing genetically modified rodents using ZFN, TALEN, and CRISPR/Cas9.
ISSN:	1940-6029
DOI:	10.1007/978-1-4939-1862-1_18