Detection and Determination of Tetracyclines in Imported Frozen Shrimp by Bioassay and HPLC

An antibacterial substance was detected in frozen shrimp imported from Thailand by use of a simplified bioassay. It appeared to be a tetracycline by classified bioassay, but we could not distinguish between tetracycline (TC) and oxytetracycline (OTC) by micro-bioautography. We were able to identify...

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Bibliographic Details
Published in:	Food Hygiene and Safety Science (Shokuhin Eiseigaku Zasshi) Vol. 35; no. 3; pp. 310 - 314_1
Main Authors:	KAMAKURA, Kazumasa, HASEGAWA, Masumi, KOIGUCHI, Satoshi, GOTO, Ikuo, SHIRAISHI, Syogo, HIRATA, Kenshi, YAMANA, Takayoshi, TONOGAI, Yasuhide
Format:	Journal Article
Language:	English Japanese
Published:	Japanese Society for Food Hygiene and Safety 1994
Subjects:	bioassay chlortetracycline high performance liquid chromatography (HPLC) Marine micro-bioautography oxytetracycline Penaeus photodiode array shrimp tetracycline tetracyclines
Online Access:	Get full text
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Summary:	An antibacterial substance was detected in frozen shrimp imported from Thailand by use of a simplified bioassay. It appeared to be a tetracycline by classified bioassay, but we could not distinguish between tetracycline (TC) and oxytetracycline (OTC) by micro-bioautography. We were able to identify the antibiotic as OTC by HPLC with a UV detector, fluorescence detector and photodiodearray detector. The content of OTC in the frozen shrimp was 1.90ppm. The detection limit of OTC was 0.01ppm by the fluorescence detector, and 0.2ppm through biological and physico-chemical determination.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	0015-6426 1882-1006
DOI:	10.3358/shokueishi.35.310