Silencing Near tRNA Genes Requires Nucleolar Localization

Transcription by RNA polymerase II is antagonized by the presence of a nearby tRNA gene in Saccharomyces cerevisiae. To test hypotheses concerning the mechanism of this tRNA gene-mediated (tgm) silencing, the effects of specific gene deletions were determined. The results show that the mechanism of...

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Bibliographic Details
Published in:	The Journal of biological chemistry Vol. 280; no. 10; pp. 8637 - 8639
Main Authors:	Wang, Li, Haeusler, Rebecca A., Good, Paul D., Thompson, Martin, Nagar, Sapna, Engelke, David R.
Format:	Journal Article
Language:	English
Published:	United States Elsevier Inc 11-03-2005 American Society for Biochemistry and Molecular Biology
Subjects:	Cell Nucleolus - genetics Gene Deletion Genes, Fungal Open Reading Frames RNA Interference RNA Polymerase II - metabolism RNA, Fungal - genetics RNA, Transfer - genetics Saccharomyces cerevisiae Saccharomyces cerevisiae - genetics
Online Access:	Get full text
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Summary:	Transcription by RNA polymerase II is antagonized by the presence of a nearby tRNA gene in Saccharomyces cerevisiae. To test hypotheses concerning the mechanism of this tRNA gene-mediated (tgm) silencing, the effects of specific gene deletions were determined. The results show that the mechanism of silencing near tRNA genes is fundamentally different from other forms of transcriptional silencing in yeast. Rather, tgm silencing is dependent on the ability to cluster the dispersed tRNA genes in or near the nucleolus, constituting a form of three-dimensional gene control.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	0021-9258 1083-351X
DOI:	10.1074/jbc.C500017200