Constituents from stem barks of Luehea ochrophylla Mart and evaluation of their antiparasitic, antimicrobial, and antioxidant activities

• Published in: Natural product research Vol. 31; no. 16; pp. 1948 - 1953
• Main Authors: Araújo, C. R. R., Silva, R. R., Silva, T. M., Takahashi, J. A., Sales-Junior, P. A., Dessimoni-Pinto, N. A. V., Souza-Fagundes, E. M., Romanha, A. J., Murta, S. M. F., Alcântara, A. F. C.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-08-2017; Taylor & Francis Ltd
• Subjects: Acetic acid; Animals; Anti-Infective Agents - chemistry; Anti-Infective Agents - pharmacology; Antifungal activity; antimicrobial action; antioxidant activity; Antioxidants - chemistry; Antioxidants - pharmacology; antiparasitic activity; Antiparasitic Agents - chemistry; Antiparasitic Agents - pharmacology; Ascorbic acid; Betulinic acid; Cercopithecus aethiops; Dichloromethane; Drug Evaluation, Preclinical - methods; Epicatechin; Ethanol; Ethyl acetate; Free radicals; Fungicides; Glucosides - isolation & purification; Glucosides - pharmacology; Inhibitory Concentration 50; Luehea ochrophylla; Magnetic Resonance Spectroscopy; Malvaceae - chemistry; NMR; Nuclear magnetic resonance; Pentacyclic Triterpenes - isolation & purification; Pentacyclic Triterpenes - pharmacology; Plant Bark - chemistry; Plant Extracts - chemistry; Sitosterols - isolation & purification; Sitosterols - pharmacology; Sterols - isolation & purification; Sterols - pharmacology; Triterpenes - isolation & purification; Triterpenes - pharmacology; Vero Cells; antiparasitic activity; Luehea ochrophylla; antioxidant activity; antimicrobial action
• ISSN: 1478-6419; 1478-6427
• DOI: 10.1080/14786419.2016.1266346

Luehea species are found in almost all Central and South American countries. The present work describes the phytochemical study, isolation, and structural characterisation of friedelin, β-friedelinol, lupeol, pseudotaraxasterol, β-sitosterol, betulinic acid, taraxasterol, (−)-epicatechin, β-sitosterol-3-O-β-d-glucopyranoside, and (+)-epicatechin-(4β→8)-epicatechin from stem barks of Luehea ochrophylla Mart. The structural identification of the isolated compounds was mainly performed by NMR analyses and comparison with the data from literature. These compounds were isolated for the first time in the genus Luehea, except β-sitosterol glucopyranoside, (−)-epicatechin, and lupeol. Hexane extract (HE) and dichloromethane (DF) and ethyl acetate (AF) fractions exhibited antiparasitic activity against amastigote (intracellular) and trypomastigote culture forms of Trypanosoma cruzi. The ethanol extract (EE), DF, and ethanol fraction (EF) exhibited considerable antifungal activity against Candida albicans. Moreover, extracts and fractions exhibited significant percentage of capture free radicals of 2,2-diphenyl-picrylhydrazyl (DPPH) when compared to the standard of ascorbic acid.