Exploring the functionalization of Ti-6Al-4V alloy with the novel antimicrobial peptide JIChis-2 via plasma polymerization

• Published in: Biofouling (Chur, Switzerland) Vol. 39; no. 1; pp. 47 - 63
• Main Authors: Teixeira, Gabriella Teresinha Lima, Gelamo, Rogério Valentim, Mateus Santos Obata, Malu, Andrade Silva, Leonardo Eurípedes de, Silva, Marcos Vinícius da, Oliveira, Carlo José Freire de, Silva, Brunela Pereira da, Aoki, Idalina Vieira, Moreto, Jeferson Aparecido, Slade, Natália Bueno Leite
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 2023; Taylor & Francis Ltd
• Subjects: Acrylic acid; Alloys; Alloys - chemistry; Alloys - pharmacology; Antiinfectives and antibacterials; Antimicrobial activity; antimicrobial peptide; Antimicrobial Peptides; Biofilms; biomedical applications; Chemical vapor deposition; Corrosion; Corrosion effects; Covalent bonds; Disease resistance; E coli; Escherichia coli; Functional materials; Gram-negative bacteria; Immobilization; Localized corrosion; Materials Testing; N-Terminus; Peptide immobilization; Peptides; plasma polymerization; Polymerization; Surface treatment; Ti-6Al-4V alloy; Titanium - chemistry; Titanium - pharmacology; Titanium base alloys; Peptide immobilization; plasma polymerization; Ti-6Al-4V alloy; antimicrobial peptide; biomedical applications
• ISSN: 0892-7014; 1029-2454
• DOI: 10.1080/08927014.2023.2183121

This study aimed to characterize the immobilization of the novel JIChis-2 peptide on the Ti-6Al-4V alloy, widely used in the biomedical sector. The antimicrobial activity of JIChis-2 was evaluated in the Gram-negative bacterium E. coli. Its immobilization occurred by inducing the formation of covalent bonds between the N-terminus of the peptides and the surface previously submitted to acrylic acid polymerization via the PECVD technique. Coated and uncoated surfaces were characterized by FTIR, AFM, SEM and EDX. Studies of global and localized corrosion were carried out, seeking to explore the effects triggered by surface treatment in an aggressive environment. Additionally, the ability of the functionalized material to prevent E. coli biofilm formation evidenced that the strategy to immobilize JIChis-2 in the Ti-6Al-4V alloy via PECVD of acrylic acid resulted in the development of a functional material with antibiofilm properties.