Expression of tumor necrosis factor alpha and interleukin 1β in jejuna of volunteers after experimental challenge with Cryptosporidium parvum correlates with exposure but not with symptoms

• Published in: Infection and immunity Vol. 69; no. 2; pp. 1172 - 1174
• Main Authors: ROBINSON, Prema, OKHUYSEN, Pablo C, CHAPPELL, Cynthia L, LEWIS, Dorothy E, SHAHAB, Imran, JANECKI, Andrzej, WHITE, A. Clinton
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-02-2001
• Series: Note
• Subjects: Animals; Bacterial diseases; Bacteriology; Biological and medical sciences; Biopsy; Cryptosporidiosis - immunology; Cryptosporidium parvum; Diarrhea - etiology; Experimental bacterial diseases and models; Fundamental and applied biological sciences. Psychology; Fungal and Parasitic Infections; Genetics; Humans; Infectious diseases; Interleukin-1 - biosynthesis; Interleukin-1 - genetics; Interleukin-15 - biosynthesis; Jejunum - metabolism; Medical sciences; Microbiology; RNA, Messenger - analysis; Tumor Necrosis Factor-alpha - biosynthesis; Tumor Necrosis Factor-alpha - genetics; Human; Protozoa; Apicomplexa; Protozoal disease; Correlation; Cytokine; Diarrhea; Interleukin 1β; Parasitosis; Necrosis; Infection; Cryptosporidiosis; Symptomatology; Messenger RNA; Cryptosporidium parvum; Biopsy; Tumor necrosis factor; Digestive diseases; Intestinal disease; Tumor; Tumor necrosis factor α
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.69.2.1172-1174.2001

Jejunal biopsies from volunteers challenged with Cryptosporidium parvum were examined for tumor necrosis factor alpha (TNF-alpha) and interleukin (IL)-1 beta mRNA. Postchallenge biopsies from 15 of 28 (54%) volunteers expressed TNF-alpha; 14% expressed IL-1 beta. Cytokine expression did not correlate with enteric symptoms, suggesting that TNF-alpha and IL-1 beta are not key mediators of diarrhea in human cryptosporidiosis.