Suppressor T cells regulating the cell-mediated immune response to Pseudomonas aeruginosa can be generated by immunization with anti- bacterial T cells

• Published in: The Journal of immunology (1950) Vol. 141; no. 11; pp. 3975 - 3979
• Main Authors: Markham, RB, Pier, GB, Powderly, WG
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Assoc Immnol 01-12-1988; American Association of Immunologists
• Subjects: Animals; Antigens, Bacterial - immunology; Antigens, Ly; Biological and medical sciences; Epitopes - immunology; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; H-2 Antigens - immunology; Immunity, Cellular - drug effects; Immunization, Passive - methods; Immunobiology; Lymphocyte Activation - drug effects; Mice; Mice, Inbred BALB C; Modulation of the immune response (stimulation, suppression); Phenotype; Pseudomonas aeruginosa; Pseudomonas aeruginosa - immunology; T-Lymphocytes, Regulatory - classification; T-Lymphocytes, Regulatory - immunology; T-Lymphocytes, Regulatory - transplantation; Vinblastine - pharmacology; Pseudomonadales; Immune response; T-Lymphocyte; Bacteria; Pseudomonadaceae; Suppressive cell; Cellular immunity; Immune regulation
• ISSN: 0022-1767; 1550-6606
• DOI: 10.4049/jimmunol.141.11.3975

We previously demonstrated that immunization with low (10 micrograms) doses of high m.w. polysaccharide from the gram-negative bacterium Pseudomonas aeruginosa generates T cells that suppress the ability of antibacterial T cells (Tab) to protect against bacterial infection. The current studies indicate that Ts cells with properties identical to those elicited by low dose polysaccharide immunization can be generated by immunization with Tab. Tab-elicited Ts cells can abrogate in vivo induction and in vitro and in vivo expression of antibacterial T cell activity. Tab-elicited Ts are Ag-specific and H-2 restricted in their suppressor activity. Non-immune T cells fail to elicit suppressor activity. These studies provide additional evidence that the protective T cell response to P. aeruginosa is controlled by a network of T cells that are probably recognizing idiotypic determinants on P. aeruginosa-immune T and B cells.