Evolutionary Profile of Mayaro Virus in the Americas: An Update into Genome Variability

• Published in: Viruses Vol. 16; no. 5; p. 809
• Main Authors: Marinho, Mikaela Dos Santos, Ferreira, Giulia Magalhães, Grosche, Victória Riquena, Nicolau-Junior, Nilson, Campos, Túlio de Lima, Santos, Igor Andrade, Jardim, Ana Carolina Gomes
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 20-05-2024
• Subjects: Adaptability; Alphavirus - classification; Alphavirus - genetics; Alphavirus - isolation & purification; Alphavirus Infections - epidemiology; Alphavirus Infections - virology; Americas - epidemiology; Amino acids; Analysis; Animals; arboviruses; E2 protein; Epidemiology; Evolution & development; Evolution, Molecular; Fever; Genetic aspects; Genetic Variation; Genome, Viral; Genomes; genomic variability; Genomics; Genotype; Genotype & phenotype; Genotypes; Homologous recombination; Humans; Hybrids; Insertion; Mayaro fever; Mayaro virus; NSP3 protein; phylogenetic analysis; Phylogenetics; Phylogeny; Proteins; Recombination, Genetic; Viruses; Americas; Brazil; Haiti; Trinidad & Tobago; United States > US; Peru; Mayaro fever; genomic variability; phylogenetic analysis; Mayaro virus; arboviruses
• ISSN: 1999-4915; 1999-4915
• DOI: 10.3390/v16050809

The Mayaro virus (MAYV) is an arbovirus with emerging potential, though with a limited understanding of its epidemiology and evolution due to the lack of studies and surveillance. Here, we investigated 71 MAYV genome sequences from the Americas available at GenBank and characterized the phylogenetic relationship among virus strains. A phylogenetic analysis showed that sequences were grouped according to the genotypes L, D, and N. Genotype D sequences were closely related to sequences collected in adjacent years and from their respective countries, suggesting that isolates may have originated from circulating lineages. The coalescent analysis demonstrated similar results, indicating the continuous circulation of the virus between countries as well. An unidentified sequence from the USA was grouped with genotype D, suggesting the insertion of this genotype in the country. Furthermore, the recombination analysis detected homologous and three heterologous hybrids which presented an insertion into the nsP3 protein. Amino acid substitutions among sequences indicated selective pressure sites, suggesting viral adaptability. This also impacted the binding affinity between the E1-E2 protein complex and the Mxra8 receptor, associated with MAYV entry into human cells. These results provide information for a better understanding of genotypes circulating in the Americas.