Decreased expression of FcγRIII mRNA in leukemic granulocytes

• Published in: FEBS letters Vol. 300; no. 1; pp. 105 - 107
• Main Authors: Monteiro, Joanita, Advani, Suresh, Gothoskar, Balwant, Zingde, Surekha
• Format: Journal Article
• Language: English
• Published: Amsterdam Elsevier B.V 23-03-1992; Elsevier
• Subjects: Biological and medical sciences; Blotting, Northern; chronic myeloid leukemia; CML; FcγRIII; Gene Expression Regulation; Granulocyte; Granulocytes - metabolism; Humans; Leukemia, Myelogenous, Chronic, BCR-ABL Positive; Leukemic; Medical sciences; Ophthalmology; paroxysmal nocturnal hemoglobinuria; phosphatidyl-inositol; PNH; Receptors, Fc - genetics; Receptors, Fc - metabolism; Reference Values; RNA, Messenger - metabolism; Tumors and pseudotumors of the eye, orbit, eyelid, lacrimal apparatus; Leukemic; PI; CML; PNH; FcγRIII; Granulocyte; Human; Membrane receptor; Messenger RNA; Fc-Fragment; Chronic myelocytic leukemia; Hemopathy; Malignant tumor; Comparative study; Quantitative analysis
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/0014-5793(92)80174-F

Morphologically mature granulocytes from patients with chronic myeloid leukemia show significant impairment in their ability to internalize aggregated IgG, a ligand that is rapidly phagocytosed by normal human granulocytes. With a view to understand the molecular basis of this defect, normal and leukemic granulocytes were examined for the steady-state levels of mRNA for FcγRIII, a membrane-associated receptor that initially binds and traps the IgG-opsonized antigens. Northern blot analyses revealed that the level of the specific mRNA in CML granulocytes was between 0.08 and 0.69 times that seen in the normal granulocytes. This could be one of the contributory factors for the observed endocytic defect in the leukemic granulocytes.