Comparison of different extraction procedures for the comprehensive characterization of bioactive phenolic compounds in Rosmarinus officinalis by reversed-phase high-performance liquid chromatography with diode array detection coupled to electrospray time-of-flight mass spectrometry

• Published in: Journal of Chromatography A Vol. 1218; no. 42; pp. 7682 - 7690
• Main Authors: Borrás Linares, I., Arráez-Román, D., Herrero, M., Ibáñez, E., Segura-Carretero, A., Fernández-Gutiérrez, A.
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 21-10-2011
• Subjects: Chromatography, High Pressure Liquid; Chromatography, Reverse-Phase - methods; Chromatography, Supercritical Fluid - methods; Flavonoids; High-performance liquid chromatography; Liquid-Liquid Extraction - methods; Mass Spectrometry - methods; Phenolic compounds; Phenols - analysis; Phenols - isolation & purification; Plant Extracts - chemistry; Plant Extracts - isolation & purification; Plant Leaves - chemistry; Pressurized liquid extraction; Rosmarinus - chemistry; Rosmarinus officinalis; Spectrophotometry, Ultraviolet; Supercritical fluid extraction; Terpenes; Time-of-flight mass spectrometry; Pressurized liquid extraction; Phenolic compounds; High-performance liquid chromatography; Supercritical fluid extraction; Time-of-flight mass spectrometry; Rosmarinus officinalis
• ISSN: 0021-9673; 1873-3778
• DOI: 10.1016/j.chroma.2011.07.021

In the present work, a comparative study between two environmentally friendly and selective extraction techniques, such as supercritical fluid extraction (SFE) and pressurized liquid extraction (PLE) have been carried out focusing in the bioactive phenolic compounds present in
Rosmarinus officinalis. For the analysis of the SFE and PLE extracts, a new methodology for qualitative characterization has been developed, based on the use of reversed-phase high-performance liquid chromatography (RP-HPLC), equipped with two different detection systems coupled in series: diode array detector (DAD) and time of flight mass spectrometry (TOF-MS) detector connected via an electrospray ionization interface (ESI). The use of a small particle size C
18 column (1.8
μm) provided a great resolution and made possible the separation of several isomers. Moreover, UV–visible spectrophotometry is a valuable tool for identifying the class of phenolic compounds, whereas MS data enabled to structurally characterize the compounds present in the extracts. The applied methodology was useful for the determination of many well-known phenolic compounds present in
R. officinalis, such as carnosol, carnosic acid, rosmadial, rosmanol, genkwanin, homoplantaginin, scutellarein, cirsimaritin and rosmarinic acid, as well as other phenolic compounds present in other species belonging to Lamiaceae family.