Detection and Tracking of Dual-Labeled HIV Particles Using Wide-Field Live Cell Imaging to Follow Viral Core Integrity

Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid p...

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Bibliographic Details
Published in:	Methods in molecular biology (Clifton, N.J.) Vol. 1354; p. 49
Main Authors:	Mamede, João I, Hope, Thomas J
Format:	Journal Article
Language:	English
Published:	United States 2016
Subjects:	Cell Culture Techniques - methods Cell Line HIV Infections - pathology HIV Infections - virology HIV-1 - isolation & purification HIV-1 - physiology Humans Microscopy - methods Optical Imaging - methods Software Staining and Labeling - methods Virus Uncoating HIV fusion HIV uncoating HIV viral core HIV early steps of infection Wide-field microscopy Live cell imaging
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Summary:	Live cell imaging is a valuable technique that allows the characterization of the dynamic processes of the HIV-1 life cycle. Here, we present a method of production and imaging of dual-labeled HIV viral particles that allows the visualization of two events. Varying release of the intravirion fluid phase marker reveals virion fusion and the loss of the integrity of HIV viral cores with the use of live wide-field fluorescent microscopy.
ISSN:	1940-6029
DOI:	10.1007/978-1-4939-3046-3_4