Accelerated follicle growth during the culture of isolated caprine preantral follicles is detrimental to follicular survival and oocyte meiotic resumption

• Published in: Theriogenology Vol. 86; no. 6; pp. 1530 - 1540
• Main Authors: Apolloni, Livia Brunetti, Bruno, Jamily Bezerra, Alves, Benner Geraldo, Ferreira, Anna Clara Accioly, Paes, Victor Macêdo, Moreno, Jesus de los Reyes Cadenas, de Aguiar, Francisco Léo Nascimento, Brandão, Felipe Zandonadi, Smitz, Johan, Apgar, Gary, de Figueiredo, José Ricardo
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-10-2016
• Subjects: Androgens; Androstenedione - biosynthesis; Androstenedione - pharmacology; Animals; Caprine; Cattle; Culture Media; Estradiol - biosynthesis; Female; Follicle Stimulating Hormone - administration & dosage; Follicle Stimulating Hormone - analysis; FSH; Goats; Growth rates; In Vitro Oocyte Maturation Techniques - veterinary; Meiosis - drug effects; Meiosis - physiology; Oocytes - cytology; Ovarian Follicle - drug effects; Ovarian Follicle - growth & development; Ovarian Follicle - metabolism; Recombinant Proteins; Secondary follicles; Tissue Culture Techniques - veterinary; Growth rates; Androgens; FSH; Secondary follicles; Caprine
• ISSN: 0093-691X; 1879-3231
• DOI: 10.1016/j.theriogenology.2016.05.012

This study investigated the effect of androstenedione (A4) alone or in association with different concentrations of bovine recombinant FSH on the IVC of isolated goat preantral follicles. Follicles were mechanically isolated from ovarian tissue and cultured for 18 days in α–minimum essential medium supplemented or not with A4 (10 ng/mL) alone or in association with fixed (A4 + FixFSH: 100 ng/mL) or sequential (A4 + SeqFSH: Day 0, 100 ng/mL; Day 6, 500 ng/mL; Day 12, 1000 ng/mL) concentrations of FSH. After 18 days, the oocytes were recovered for IVM and fluorescence analysis. At Day 18 of culture, only A4 + SeqFSH treatment showed a lower (P < 0.05) rate of intact follicles, survival probability, and meiotic resumption, as well as higher (P < 0.05) percentage of degeneration and/or extrusion after antrum formation. Taken together, these results reported a positive correlation between fast-growing follicles and follicles that degenerated and/or extruded after antrum formation. When compared with control, the addition of A4 alone or in association of FSH did not increase (P > 0.05) the estradiol production or androstenedione levels on Day 6. However, on Day 18, the androstenedione levels were significantly lower in A4 + SeqFSH treatment when compared with A4 alone or to A4 + FixFSH treatments, whereas the estradiol production did not differ (P > 0.05). In summary, this study found that accelerated follicle growth negatively impacted the morphology of caprine preantral follicle cultured in vitro. In addition, the association of androstenedione with increasing concentration of FSH was detrimental to follicular survival and oocyte meiotic resumption.