An improved method to distinguish Entamoeba histolytica and Entamoeba dispar

A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. The amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alt...

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Bibliographic Details
Published in:	Parasitology Vol. 119 ( Pt 4); p. 359
Main Authors:	Gomes, M A, Pesquero, J B, Furst, C, Valle, P R, Pesquero, J L, Silva, E F
Format:	Journal Article
Language:	English
Published:	England 01-10-1999
Subjects:	Animals Entamoeba - classification Entamoeba - genetics Entamoeba histolytica - classification Entamoeba histolytica - genetics Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length Sequence Analysis, DNA
Online Access:	Get more information
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Summary:	A 482 base pair gene fragment from samples of amoebae E. histolytica and E. dispar was amplified by PCR. The amplification products of fragments from the 2 species of amoebae presented differences in mobility in non-denaturing polyacrylamide gel, probably due to sequence-dependent conformational alterations in the DNA fragments. The method described here permits E. histolytica and E. dispar to be distinguished with greater sensitivity and rapidity.
ISSN:	0031-1820
DOI:	10.1017/S0031182099004886