Genome Sequence and Characterization of Coliphage vB_Eco_SLUR29

• Published in: PHAGE (Print) Vol. 1; no. 1; pp. 38 - 44
• Main Authors: Besler, Ibrahim, Sazinas, Pavelas, Harrison, Christian, Gannon, Lucy, Redgwell, Tamsin, Michniewski, Slawomir, Hooton, Steven P, Hobman, Jon L, Millard, Andrew
• Format: Journal Article
• Language: English
• Published: United States Mary Ann Liebert, Inc., publishers 01-03-2020
• Subjects: Original; Original Articles; coliphage; genomes; phages
• ISSN: 2641-6530; 2641-6549; 2641-6549
• DOI: 10.1089/phage.2019.0009

Background:
Bacteriophages that infect
Escherichia coli
are relatively easily isolated, with >600 coliphage genomes sequenced to date. Despite this there is still much to be discovered about the diversity of coliphage genomes.
Materials and Methods:
Within this study, we isolated a coliphage from cattle slurry collected from a farm in rural England.
Results:
Transmission electron microscopy identified the phage as member of the
Siphoviridae
family. Phylogenetic analysis and comparative genomics further placed it within the subfamily
Tunavirinae
and forms part of a new genus.
Conclusions:
Characterization of the lytic properties of vB_Eco_SLUR29 reveals that it is rapidly able to lyse its host when infected at high multiplicity of infection, but not at low multiplicity of infection.