Plasmonic DNA hotspots made from tungsten disulfide nanosheets and gold nanoparticles for ultrasensitive aptamer-based SERS detection of myoglobin

• Published in: Mikrochimica acta (1966) Vol. 185; no. 3; pp. 158 - 8
• Main Authors: Shorie, Munish, Kumar, Vinod, Kaur, Harmanjit, Singh, Kulvinder, Tomer, Vijay K., Sabherwal, Priyanka
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-03-2018; Springer; Springer Nature B.V
• Subjects: Analytical Chemistry; Characterization and Evaluation of Materials; Chemistry; Chemistry and Materials Science; Deoxyribonucleic acid; DNA; Electromagnetic properties; Electromagnetism; Gold; Lasers; Microengineering; Myoglobin; Myoglobins; Nanochemistry; Nanoparticles; Nanosheets; Nanotechnology; Original Paper; Substrates; Tungsten; Tungsten compounds; Tungsten disulfide; Cardiac biomarker; SERS aptasensor; Plasmonic hotspots; Nanohybrid; SELEX
• ISSN: 0026-3672; 1436-5073
• DOI: 10.1007/s00604-018-2705-x

A nanohybrid mediated SERS substrate was prepared by
in-situ
synthesis and assembly of gold nanoparticles (AuNPs) on exfoliated nanosheets of tungsten disulfide (WS
2
) to form plasmonic hotspots. The nanohybrid surface was functionalized with specific aptamers which imparted high selectivity for the cardiac marker myoglobin (Mb). The fabricated aptasensor was read by SERS using a 532 nm laser and demonstrated significant signal enhancement, and this allowed Mb to be determined in the 10 f. mL
−1
to 0.1 μg mL
−1
concentration range. The study presents an approach to synergistically exploit the unique chemical and electromagnetic properties of both WS
2
and AuNPs for many-fold enhancement of SERS signals.
Graphical abstract
Schematic presentation of a nanohybrid-mediated SERS substrate prepared by
in-situ
assembly of gold nanoparticles (AuNPs) reduced on exfoliated nanosheets of tungsten disulfide (WS
2
) to form plasmonic hot spots. Specific aptamers immobilized on the SERS surface impart high sensitivity and selectivity for the cardiac marker myoglobin (Mb).