Single Cell Proteogenomics — Immediate Prospects

• Published in: Biochemistry (Moscow) Vol. 85; no. 2; pp. 140 - 146
• Main Authors: Moshkovskii, S. A., Lobas, A. A., Gorshkov, M. V.
• Format: Journal Article
• Language: English
• Published: Moscow Pleiades Publishing 01-02-2020; Springer; Springer Nature B.V
• Subjects: Analysis; Animals; Biochemistry; Biomedical and Life Sciences; Biomedicine; Bioorganic Chemistry; Cell interactions; Chromatography, Liquid; Gene expression; Genetic aspects; Genomics; Humans; Innovations; Life Sciences; Liquid chromatography; Mass Spectrometry; Mass spectroscopy; Microbiology; Proteogenomics; Proteomics; Review; Scientific imaging; Single-Cell Analysis; Spectroscopy; Unicellular organisms; Russia; tandem mass tag (TMT); transcriptomics; mass spectrometry; proteomics; single cell analysis; proteogenomics
• ISSN: 0006-2979; 1608-3040
• DOI: 10.1134/S0006297920020029

Recent technical advances in genomic technology have led to the explosive growth of transcriptome-wide studies at the level of single cells. The review describes the first steps of the single cell proteomics that has originated soon after development of transcriptomics methods. The first studies on the shotgun proteomics of single cells that used liquid chromatography/mass spectrometry have been already published. In these works, the cells were separated by the methods used in transcriptomics studies (e.g., cell sorting) and analyzed by modified mass spectrometry with tandem mass tags. The new proteogenomics approach involving integration of single cell transcriptomics and proteomics data will provide better understanding of the mechanisms of cell interactions in normal development and disease.