Cefquinome-Loaded Microsphere Formulations in Protection against Pneumonia with Klebsiella pneumonia Infection and Inflammatory Response in Rats

• Published in: Pharmaceutical research Vol. 36; no. 5; pp. 74 - 10
• Main Authors: Qu, Shaoqi, Dai, Cunchun, Yang, Fenfang, Huang, Tingting, Hao, Zhihui, Tang, Qihe, Wang, Haixia, Zhang, Yanping
• Format: Journal Article
• Language: English
• Published: New York Springer US 01-05-2019; Springer; Springer Nature B.V
• Subjects: Alveoli; Bacterial pneumonia; Biochemistry; Biomedical and Life Sciences; Biomedical Engineering and Bioengineering; Biomedicine; Body weight; Bronchus; Formulations; Glycolic acid; Health aspects; Infections; Inflammation; Inflammatory response; Interleukin 4; Interleukin 6; Interleukins; Klebsiella; Lungs; Medical Law; Medical research; Medicine, Experimental; Microspheres; Pharmacology/Toxicology; Pharmacy; Pneumonia; Polylactide-co-glycolide; Research Paper; Rodents; Tumor necrosis factor-TNF; Tumor necrosis factor-α; cefquinome; inflammation; pharmacodynamics; poly lactic-co-glycolic acid microspheres; Klebsiella pneumonia
• ISSN: 0724-8741; 1573-904X
• DOI: 10.1007/s11095-019-2614-7

Purpose
This study aimed to compare
in vivo
activity between cefquinome (CEQ)-loaded poly lactic-co-glycolic acid (PLGA) microspheres (CEQ-PLGA-MS) and CEQ injection (CEQ-INJ) against
Klebsiella pneumonia
in a rat lung infection model.
Methods
Forty-eight
rats were divided into control group (sham operated without infection and drug treatment),
Klebsiella pneumonia
model group (KPD + Saline), CEQ-PLGA-MS and CEQ-INJ therapy groups (KPD + CEQ-PLGA-MS and KPD + INJ, respectively). In the KPD + Saline group, rats were infected with
Klebsiella pneumonia
ATCC 10031. In the KPD + CEQ-PLGA-MS and KPD + INJ groups, infected rats were intravenously injected with 12.5 mg/kg body weight CEQ-PLGA-MS and CEQ-INJ, respectively.
Results
Compared to CEQ-INJ treatment group, CEQ-PLGA-MS treatment further decreased the number of bacteria colonies (decreased to 1.94 lg CFU/g) in lung tissues and the levels of inflammatory cytokine including tumor necrosis factor (TNF)-α, interleukin (IL)-1β, IL-6, IL-4 (
p
 < 0.05 or
p
 < 0.01) in bronchoalveolar lavage fluid at 48 h. Consistently, a significant decreases of scores of inflammation severity were showed at 48 h in the KPD + CEQ-PLGA-MS treatment group, compared to the KPD + CEQ-INJ treatment group.
Conclusion
Our results reveal that CEQ-PLGA-MS has the better therapeutic effect than CEQ-INJ for
Klebsiella pneumonia
lung infections in rats. The vehicle of CEQ-PLGA-MS as the promising alternatives to control the lung infections with the important pathogens.