Physiological characterization of the wild almond Prunus arabica stem photosynthetic capability

• Published in: Frontiers in plant science Vol. 13; p. 941504
• Main Authors: Trainin, Taly, Brukental, Hillel, Shapira, Or, Attia, Ziv, Tiwari, Vivekanand, Hatib, Kamel, Gal, Shira, Zemach, Hanita, Belausov, Eduard, Charuvi, Dana, Holland, Doron, Azoulay-Shemer, Tamar
• Format: Journal Article
• Language: English
• Published: Frontiers Media S.A 29-07-2022
• Subjects: almond; CO2-assimilation; Plant Science; stem photosynthetic capabilities (SPC); stem recycling photosynthesis (SRP); stomata; transpiration
• ISSN: 1664-462X; 1664-462X
• DOI: 10.3389/fpls.2022.941504

Leaves are the major plant tissue for transpiration and carbon fixation in deciduous trees. In harsh habitats, atmospheric CO
2
assimilation
via
stem photosynthesis is common, providing extra carbon gain to cope with the detrimental conditions. We studied two almond species, the commercial
Prunus dulcis
cultivar “Um-el-Fahem” and the rare wild
Prunus arabica
. Our study revealed two distinctive strategies for carbon gain in these almond species. While, in
P. dulcis
, leaves possess the major photosynthetic surface area, in
P. arabica
, green stems perform this function, in particular during the winter after leaf drop. These two species' anatomical and physiological comparisons show that
P. arabica
carries unique features that support stem gas exchange and high-gross photosynthetic rates
via
stem photosynthetic capabilities (SPC). On the other hand,
P. dulcis
stems contribute low gross photosynthesis levels, as they are designed solely for reassimilation of CO
2
from respiration, which is termed stem recycling photosynthesis (SRP). Results show that (a)
P. arabica
stems are covered with a high density of sunken stomata, in contrast to the stomata on
P. dulcis
stems, which disappear under a thick peridermal (bark) layer by their second year of development. (b)
P. arabica
stems contain significantly higher levels of chlorophyll compartmentalized to a mesophyll-like, chloroplast-rich, parenchyma layer, in contrast to rounded-shape cells of
P. dulcis's
stem parenchyma. (c) Pulse amplitude-modulated (PAM) fluorometry of
P. arabica
and
P. dulcis
stems revealed differences in the chlorophyll fluorescence and quenching parameters between the two species. (d) Gas exchange analysis showed that guard cells of
P. arabica
stems tightly regulate water loss under elevated temperatures while maintaining constant and high assimilation rates throughout the stem. Our data show that
P. arabica
uses a distinctive strategy for tree carbon gain
via
stem photosynthetic capability, which is regulated efficiently under harsh environmental conditions, such as elevated temperatures. These findings are highly important and can be used to develop new almond cultivars with agriculturally essential traits.