Entire nucleotide sequence and characterization of a hepatitis C virus of genotype V/3a

• Published in: Journal of general virology Vol. 75; no. 7; pp. 1761 - 1768
• Main Authors: Sakamoto, Minoru, Akahane, Yoshihiro, Tsuda, Fumio, Tanaka, Takeshi, Woodfield, D. Graeme, Okamoto, Hiroaki
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-07-1994; Society for General Microbiology
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; Carrier State; DNA, Complementary; Fundamental and applied biological sciences. Psychology; Genes, Viral - genetics; Genetic Variation - genetics; Genetics; Genome, Viral; Genotype; Hepacivirus - classification; Hepacivirus - genetics; Hepatitis C - microbiology; hepatitis C virus; Humans; Microbiology; Molecular Sequence Data; New Zealand; Nucleic Acid Conformation; Open Reading Frames; Phylogeny; Sequence Alignment; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Virology; New Zealand; Virus; Genetic mapping; Molecular evolution; Genome organization; Genetic variability; Nucleotide sequence; Genotype; Homology; Phylogeny; Flaviviridae; Hepatitis C virus; Aminoacid sequence
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-75-7-1761

1 First Department of Internal Medicine, Yamanashi Medical College, Yamanashi-Ken 409-38
2 Viral Hepatitis Research Foundation of Japan, Tokyo 113
3 Japanese Red Cross Saitama Blood Center, Saitama-Ken 338, Japan
4 Department of Transfusion Medicine, Regional Blood Center, Auckland, New Zealand
and 5 Immunology Division, Jichi Medical School, Tochigi-Ken 329-04, Japan
The entire nucleotide sequence of a hepatitis C virus (HCV) genome (NZL1) of genotype V/3a was determined from overlapping cDNA clones obtained from a human carrier in New Zealand. It comprised 9425 nucleotides (nt) including a 5'-untranslated region of 339 nt, a single large open reading frame encoding a polyprotein of 3021 amino acids, a 3'-untranslated region of 23 nt, and 3'-terminal poly(U) stretches of variable lengths. The NZL1 genome was compared with 15 HCV isolates of other genotypes for which the full-length sequence has been determined. It differed from them by 31.1 to 34.3% in nucleotide sequence identity and by 24.5 to 29.1% in amino acid sequence identity, confirming the distinction of genotype V/3a from the other isolates.
Received 29 November 1993;
accepted 12 January 1994.