Laser and light-emitting diode effects on pre-osteoblast growth and differentiation

• Published in: Lasers in medical science Vol. 29; no. 1; pp. 55 - 59
• Main Authors: Pagin, Marina Tochetti, de Oliveira, Flávia Amadeu, Oliveira, Rodrigo Cardoso, Sant’Ana, Adriana Campos Passanezi, de Rezende, Maria Lucia Rubo, Greghi, Sebastião Luiz Aguiar, Damante, Carla Andreotti
• Format: Journal Article
• Language: English
• Published: London Springer London 2014; Springer Nature B.V
• Subjects: 3T3 Cells; Acceleration; Alkaline Phosphatase - metabolism; Animals; Bone Regeneration - radiation effects; Bones; Cell Differentiation - radiation effects; Cell Proliferation - radiation effects; Density; Dentistry; Differentiation; Irradiation; Lasers; Lasers, Semiconductor - therapeutic use; Light; Light emitting diodes; Low-Level Light Therapy; Medical science; Medicine; Medicine & Public Health; Mice; Optical Devices; Optics; Original Article; Orthodontics; Osteoblasts - cytology; Osteoblasts - enzymology; Osteoblasts - radiation effects; Photonics; Phototherapy; Quantum Optics; Semiconductors; Wound healing; Semiconductor; Wound healing; Lasers; Bone regeneration; Phototherapy
• ISSN: 0268-8921; 1435-604X
• DOI: 10.1007/s10103-012-1238-5

The acceleration of bone regeneration by low-intensity laser irradiation may hold potential benefits in clinical therapy in orthopedics and dentistry. The purpose of this study is to compare the effects of light-emitting diode (LED) and laser on pre-osteoblast MC3T3 proliferation and differentiation. Cells were irradiated with red, infrared, and LED (3 and 5 J/cm
2
). Lasers had a power density of 1 W/cm
2
and irradiation time of 2 and 5 s. LED had a power density of 60 mW/cm
2
and irradiation time of 50 and 83 s. Control group did not receive irradiation. Cell growth was assessed by a colorimetric test (MTT) (24, 48, 72, and 96 h), and cell differentiation was evaluated by alkaline phosphatase (ALP) quantification after growth in osteogenic medium (72 and 96 h and 7 and 14 days). At 24 h, the cell growth was enhanced 3.6 times by LED (5 J/cm
2
), 6.8 times by red laser (3 J/cm
2
), and 10.1 times by red laser (5 J/cm
2
) in relation to control group (
p
 < 0.05). At the other periods, there was no influence of irradiation on cell growth (
p
 > 0.05). The production of ALP was not influenced by irradiation at any period of time (
p
 > 0.05). Low-intensity laser and LED have similar effects on stimulation of cell growth, but no effect on cell differentiation.