Association of TP53 codon 72 and intron 3 16-bp Ins/Del polymorphisms with cervical cancer risk

• Published in: Tumor biology Vol. 35; no. 8; pp. 7435 - 7440
• Main Authors: Laprano, Tatiana Dantas Rodrigues, Lemos, Érika Hardy, Cunha, Lia Moreira Pinto, Júnior, José Eleutério, de SousaTeles, Rosiane Alves, Rabenhorst, Silvia Helena Barem
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-08-2014; Springer Nature B.V
• Subjects: Adolescent; Adult; Biomedical and Life Sciences; Biomedicine; Cancer Research; Cervical cancer; Cervical Intraepithelial Neoplasia - genetics; Codon; Female; Genes, p53; Genetic Predisposition to Disease; Genotype & phenotype; Humans; Introns; Middle Aged; Polymorphism; Polymorphism, Genetic; Research Article; Risk; Risk factors; Uterine Cervical Neoplasms - etiology; Uterine Cervical Neoplasms - genetics; Cervical intraepithelial neoplasia (CIN); gene; HPV; Polymorphism
• ISSN: 1010-4283; 1423-0380
• DOI: 10.1007/s13277-014-1988-8

Cervical cancer incidence has grown worldwide, with it being a more significant problem in developing countries. Invasive squamous cell cervical cancers are preceded by a long phase of preinvasive disease, known as cervical intraepithelial neoplasia. Cervical cancer can develop when the virus takes advantage of any
TP53
gene dysfunction of the host organism.
TP53
is responsible for encoding the tumor suppressor p53 phosphoprotein, which helps preserve genome integrity. Currently, many studies have focused on genetic polymorphisms as an important contribution to cancer susceptibility, but few related to cervical intraepithelial neoplasia (CIN). Thus, the present study aimed to see whether patients with suspected CIN had
TP53
gene polymorphisms that might have contributed to the development of neoplasia. This study included 133 women who were referred to the Cervical Pathology Clinic of the Maternity School Assis Chateaubriand MEAC for suspected cervical lesions. Polymorphism genotyping was carried out by the PCR-RFLP technique using DNA extracted from patients’ blood. The most frequent genotype in both CIN(+) and CIN(−) patients was Arg/Pro
TP53
codon 72 and A1A1 for 16-bp Del in intron 3. No risk of cervical cancer was found for the polymorphisms studied. However, a significant association was found when the two polymorphisms were combined: patients with the A1A1/ArgPro genotype were statistically more frequent in the CIN(−) group (
p
 = 0.042), while A2A2-A1A2/ProArg was significantly more frequent in the CIN(+) group. The results of our study suggest that combined analysis of TP53 polymorphisms Arg72Pro and 16-bp Ins/Del may help to monitor the development of CIN in Brazilian women.