abaB, a putative regulator for secondary metabolism in Streptomyces

Abstract A chromosomal DNA fragment from Streptomyces antibioticus ATCC11891 was isolated by its ability to stimulate actinorhodin and undecylprodigiosin biosynthesis in Streptomyces lividans TK21. This fragment includes two open reading frames, whose deduced translated products resemble enzymes inv...

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Bibliographic Details
Published in:	FEMS microbiology letters Vol. 147; no. 1; pp. 29 - 36
Main Authors:	Scheu, A.-K., Martínez, E., Soliveri, J., Malpartida, F.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-02-1997 Oxford University Press
Subjects:	Actinorhodin Anti-Bacterial Agents - biosynthesis Antibiotic Antibiotics Base Sequence Biosynthesis Blotting, Southern Chromatography, High Pressure Liquid Cloning Cloning, Molecular Copy number Deoxyribonucleic acid DNA DNA, Fungal - genetics Gene Dosage Gene Expression Regulation, Fungal - genetics Genes, Fungal - genetics LysR Metabolism Microbiology Molecular Sequence Data Oleandomycin - biosynthesis Open reading frames Open Reading Frames - genetics Phenotypes Plasmids Pleiotropic regulator Promoter Promoter Regions, Genetic - physiology Regulators Restriction Mapping Sequence Analysis, DNA Sequence Homology, Amino Acid Streptomyces Streptomyces - genetics Streptomyces - metabolism Streptomyces antibioticus Streptomyces lividans Streptomycetes Sulfur Titration Transcription Transcription, Genetic - genetics Undecylprodigiosin Promoter LysR Pleiotropic regulator Antibiotic Actinorhodin
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Summary:	Abstract A chromosomal DNA fragment from Streptomyces antibioticus ATCC11891 was isolated by its ability to stimulate actinorhodin and undecylprodigiosin biosynthesis in Streptomyces lividans TK21. This fragment includes two open reading frames, whose deduced translated products resemble enzymes involved in sulfur metabolism (ORF1) and LysR-type transcriptional regulators (ORF2). The cloning of the promoter region of ORF2 (abaB) in high copy number led to overproduction of both antibiotics suggesting that this phenotype might well be due to titration by this region of one or more proteins. Southern blot analysis revealed that abaB gene is highly conserved among all streptomycetes tested.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0378-1097 1574-6968
DOI:	10.1111/j.1574-6968.1997.tb10216.x