The maize actin‐depolymerizing factor, ZmADF3, redistributes to the growing tip of elongating root hairs and can be induced to translocate into the nucleus with actin

• Published in: The Plant journal : for cell and molecular biology Vol. 12; no. 5; pp. 1035 - 1043
• Main Authors: Jiang, Chang‐Jie, Weeds, Alan G., Hussey, Patrick J.
• Format: Journal Article
• Language: English
• Published: Osney Mead, Oxford OX2 0EL, UK Blackwell Science Ltd 01-11-1997; Blackwell Science
• Subjects: Actin Depolymerizing Factors; Actins - drug effects; Actins - metabolism; Biological and medical sciences; Cell biochemistry; Cell Nucleus - metabolism; Cell physiology; Cytochalasin D - pharmacology; Cytoplasm - metabolism; Destrin; Fundamental and applied biological sciences. Psychology; Kinetics; Microfilament Proteins - metabolism; Plant physiology and development; Plant Roots - physiology; Recombinant Proteins - metabolism; Zea mays - physiology; Monocotyledones; Zea mays; Root; Root hair; Polymerization; Depolymerization; Regulation(control); Gramineae; Actin; Dynamics; Angiospermae; Distribution; Development; Cytoskeleton; Spermatophyta; Cytoplasm
• ISSN: 0960-7412; 1365-313X
• DOI: 10.1046/j.1365-313X.1997.12051035.x

Summary
The maize actin depolymerizing factor, ZmADF3, binds G‐and F‐actin, and increases in vitro actin dynamics. Polyclonal antibodies have been raised against ZmADF3 and these detect a single band of approximately 17 kDa in all maize tissues examined, with the exception of pollen. In the development of root hairs, the distribution of ZmADF3 is related to actin reorganization. In the early stages of hair development, ZmADF3 is distributed throughout the cytoplasm. As the hair emerges and the microfilament bundles redirect to the outgrowth there is a simultaneous redistribution of ZmADF3 which now concentrates at the tip of the emerging hair and remains in this position as elongation proceeds. These observations show that ZmADF3 localizes to a region where actin is being remodelled during tip growth. After cytochalasin D treatment which disrupts actin filaments, short rods of ZmADF3 and actin appear in the nucleus suggesting that ZmADF3 may function by guiding actin to sites of actin polymerization.