Genomic landscape of NDM-1 producing multidrug-resistant Providencia stuartii causing burn wound infections in Bangladesh

• Published in: Scientific reports Vol. 14; no. 1; pp. 2246 - 15
• Main Authors: Mondol, Spencer Mark, Islam, Israt, Islam, Md. Rafiul, Shakil, Shahriar Kabir, Rakhi, Nadira Naznin, Mustary, Jannatul Ferdous, Amiruzzaman, Gomes, Donald James, Shahjalal, Hussain Md, Rahaman, Md. Mizanur
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 26-01-2024; Nature Publishing Group; Nature Portfolio
• Subjects: 631/114; 631/114/2785; 631/326; 631/326/41/2530; Anti-Bacterial Agents - pharmacology; Anti-Bacterial Agents - therapeutic use; Antibiotics; Antimicrobial agents; Antimicrobial resistance; Bangladesh; beta-Lactamases - genetics; Biofilms; Burn patients; Burns - drug therapy; Carbapenems; Communicable Diseases - drug therapy; Drug resistance; Drug Resistance, Multiple, Bacterial - genetics; Enterobacteriaceae Infections - drug therapy; Enterobacteriaceae Infections - epidemiology; Genetic diversity; Genomes; Genomics; Humanities and Social Sciences; Humans; Microbial Sensitivity Tests; multidisciplinary; Multidrug resistance; Multidrug resistant organisms; Opportunist infection; Pathogens; Plasmids; Providencia; Providencia stuartii; Public health; Science; Science (multidisciplinary); Wound infection; Wound Infection - drug therapy; β Lactamase; Bangladesh
• ISSN: 2045-2322; 2045-2322
• DOI: 10.1038/s41598-024-51819-9

The increasing antimicrobial resistance in
Providencia stuartii
(
P. stuartii
) worldwide, particularly concerning for immunocompromised and burn patients, has raised concern in Bangladesh, where the significance of this infectious opportunistic pathogen had been previously overlooked, prompting a need for investigation
.
The two strains of
P. stuartii
(
P. stuartii
SHNIBPS63
and P. stuartii
SHNIBPS71) isolated from wound swab of two critically injured burn patients were found to be multidrug-resistant and
P. stuartii
SHNIBPS63 showed resistance to all the 22 antibiotics tested as well as revealed the co-existence of
bla
VEB-6
(Class A),
bla
NDM-1
(Class B),
bla
OXA-10
(Class D) beta lactamase genes. Complete resistance to carbapenems through the production of NDM-1, is indicative of an alarming situation as carbapenems are considered to be the last line antibiotic to combat this pathogen. Both isolates displayed strong biofilm-forming abilities and exhibited resistance to copper, zinc, and iron, in addition to carrying multiple genes associated with metal resistance and the formation of biofilms. The study also encompassed a pangenome analysis utilizing a dataset of eighty-six publicly available
P. stuartii
genomes (n = 86), revealing evidence of an open or expanding pangenome for
P. stuartii
. Also, an extensive genome-wide analysis of all the
P. stuartii
genomes revealed a concerning global prevalence of diverse antimicrobial resistance genes, with a particular alarm raised over the abundance of carbapenem resistance gene
bla
NDM-1
. Additionally, this study highlighted the notable genetic diversity within
P. stuartii
, significant informations about phylogenomic relationships and ancestry, as well as potential for cross-species transmission, raising important implications for public health and microbial adaptation across different environments.