Flow-Cytometric Detection of Terminal Deoxynucleotidyl Transferase and Other Intracellular Antigens in Combination With Membrane Antigens in Acute Lymphatic Leukemias

Development of a new fixation procedure allowed flow-cytometric analysis of nuclear and other intracellular antigens in acute lymphatic leukemia (ALL). A short fixation of the cells with buffered formaldehyde acetone (BFA) rendered the cell membrane permeable, allowing the monoclonal antibodies (MoA...

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Bibliographic Details
Published in:	Blood Vol. 72; no. 5; pp. 1639 - 1644
Main Authors:	Slaper-Cortenbach, Ineke CM, Admiraal, Lucia G., Kerr, Jane M., Leeuwen, Eleonore F. van, Borne, Albert E.G. Kr. von dem, Tetteroo, Pedro A.T.
Format:	Journal Article
Language:	English
Published:	Washington, DC Elsevier Inc 01-11-1988 The Americain Society of Hematology
Subjects:	Antigens - analysis Antigens, Differentiation - analysis Antigens, Neoplasm - analysis Antigens, Surface - analysis Biological and medical sciences Biomarkers, Tumor - analysis Cell Membrane Permeability DNA Nucleotidylexotransferase - blood Fixatives Flow Cytometry Fluorescent Antibody Technique Hematologic and hematopoietic diseases Humans Leukemia, Lymphocytic, Chronic, B-Cell - enzymology Leukemia, Lymphocytic, Chronic, B-Cell - immunology Leukemias. Malignant lymphomas. Malignant reticulosis. Myelofibrosis Medical sciences Precursor Cell Lymphoblastic Leukemia-Lymphoma - enzymology Precursor Cell Lymphoblastic Leukemia-Lymphoma - immunology Human Flow cytometry Residual tumor Histological preparation Hemopathy Monoclonal antibody Antigen DNA nucleotidylexotransferase Screening Plasma membrane Acute lymphocytic leukemia Immunofluorescence Cytoplasm
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