Studies on the oral absorption of cyclic peptides from Pseudostellaria heterophylla and interacting with membrane receptors

• Published in: Fitoterapia Vol. 156; p. 105072
• Main Authors: Zhao, Li, Yang, Han, Jiang, Chang, Kan, Yongjun, Hu, Juan, Pang, Wensheng
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier B.V 01-01-2022; Elsevier Science Ltd
• Subjects: Absorption; Animals; Caryophyllaceae - chemistry; Chromatography, High Pressure Liquid; Cyclic peptides; Drug development; Herbal medicine; High-performance liquid chromatography; In vivo methods and tests; Liquid chromatography; Male; Mass Spectrometry; Mass spectroscopy; Membrane receptors; Membranes; Monomers; Oral absorption mechanism; Oral administration; Peptides; Peptides, Cyclic - chemistry; Peptides, Cyclic - isolation & purification; Peptides, Cyclic - metabolism; Pseudostellaria; Pseudostellaria heterophylla; R&D; Rats; Receptors; Research & development; Surface plasmon resonance; Traditional Chinese medicine; UPLC-MS/MS; Pseudostellaria heterophylla; UPLC-MS/MS; Oral absorption mechanism; Surface plasmon resonance; Membrane receptors; Cyclic peptides
• ISSN: 0367-326X; 1873-6971
• DOI: 10.1016/j.fitote.2021.105072

Pseudostellaria heterophylla (Miq.) Pax. (Taizishen, TZS) contains a variety of natural active cyclic-peptide compounds (CP). In this article, four kinds of CP monomers were isolated by HPLC and the structures were identified by mass spectrometry. The in vivo absorption of CP was detected by UPLC-MS/MS. The interaction between CP and membrane receptor was analyzed by SPR. As a result, the relative absorption rate of CP was Pesudostellarin B > Heterophyllin B > Pesudostellarin C > Pesudostellarin E. The difference in absorption rate of CP in vivo was related to its interaction with membrane receptors. The absorption mechanism of CP might be different. This is the first report that in vivo absorption study of different CP from TZS and explore its absorption mechanism, laying a theoretical foundation for the research and development of its oral drugs, and providing new ideas for the study of the absorption mechanism of CP from traditional Chinese medicine.
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•Four kinds of Pseudostellaria heterophylla cyclic-peptide were isolated and the structures were identified.•Difference absorption rate of cyclic-peptides in vivo was related to its interaction with cell membrane receptors.•The absorption mechanism of cyclic-peptides from Pseudostellaria heterophylla might be different.