Comparative analysis of the N-genome in diploid and polyploid Aegilops species

• Published in: Chromosome research Vol. 19; no. 4; pp. 541 - 548
• Main Authors: Badaeva, Ekaterina D., Dedkova, Olga S., Zoshchuk, Svyatoslav A., Amosova, Alexandra V., Reader, Stephen M., Bernard, Michel, Zelenin, Alexander V.
• Format: Journal Article
• Language: English
• Published: Dordrecht Springer Netherlands 01-05-2011; Springer Nature B.V; Springer Verlag
• Subjects: Aegilops; Aegilops ventricosa; Agricultural sciences; Animal Genetics and Genomics; Biomedical and Life Sciences; Cell Biology; Chromosome Banding; Chromosomes; Chromosomes, Plant - genetics; Deoxyribonucleic acid; Diploidy; DNA; DNA, Ribosomal - genetics; Evolution; Fluorescence in situ hybridization; Genome, Plant - genetics; Genomics; Grasses; Human Genetics; In Situ Hybridization, Fluorescence; Karyotyping; Life Sciences; Plant Genetics and Genomics; Poaceae - genetics; Polyploidy; Tandem Repeat Sequences; Triticum aestivum; C-banding; Fluorescence in situ hybridisation; evolution; N-genome; genetic nomenclature of chromosomes; REPETITIVE DNA-SEQUENCES; IN-SITU HYBRIDIZATION; CHROMOSOMES; RIBOSOMAL-RNA GENES; WHEAT; UNIARISTATA; TRITICUM; Aegilops; EVOLUTION; DIFFERENTIATION; POACEAE
• ISSN: 0967-3849; 1573-6849
• DOI: 10.1007/s10577-011-9211-x

The genetic classification for the N-genome chromosomes has been developed on the basis of C-banding analysis on the set of
Triticum aestivum
×
Aegilops uniaristata
single chromosome addition lines and examination of
A. uniaristata
(
, NN),
Aegilops ventricosa
(
, DDNN) and
Aegilops recta
(
, UUX
n
X
n
NN) accessions carrying intergenomic translocations using fluorescence in situ hybridisation with probes for three repetitive DNA sequences as well as the 5S and 45S rDNA families. The N-genome chromosomes of the tetraploid
A. ventricosa
show significant changes relative to the diploid progenitor species, while those of the hexaploid
A. recta
are similar to
A. uniaristata
with regard to the distribution of C-bands, 45S and 5S rDNA loci and hybridisation sites of all the three families of tandem repeats. The possible mechanisms of N-genome evolution are discussed.