Interactions between bioactive components determine antioxidant, cytotoxic and nutrigenomic activity of cocoa powder extract

• Published in: Free radical biology & medicine Vol. 154; pp. 48 - 61
• Main Authors: Baranowska, Monika, Suliborska, Klaudia, Todorovic, Vanja, Kusznierewicz, Barbara, Chrzanowski, Wojciech, Sobajic, Sladjana, Bartoszek, Agnieszka
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-07-2020
• Subjects: Antioxidants; Catechins; Chemoprevention; Cocoa powder; Food synergy; Redox biology; Antioxidants; Catechins; Redox biology; Cocoa powder; Chemoprevention; Food synergy
• ISSN: 0891-5849; 1873-4596
• DOI: 10.1016/j.freeradbiomed.2020.04.022

Numerous studies have shown, rather disappointingly, that isolated bioactive phytochemicals are not as biologically effective as natural plant products. Such a discrepancy may be explained by the concept of food synergy, which was verified in this research for cocoa extract versus its major components with regard to cancer chemoprevention. The evaluation embraced the relationship between redox properties evaluated in cell-free systems with the aid of free radicals scavenging method and differential pulse voltammetry, and redox associated anticarcinogenic activities (cellular antioxidant activity, cytotoxicity, nutrigenomic activity) in human colon adenocarcinoma cell line exposed to either cocoa powder extract or artificial mixtures of cocoa bioactives at matching concentrations. In contrast to expectations, our results showed that the stepwise enrichment with antioxidants caused no gradual increase in the antioxidant activity of the model mixtures; also, these model mixtures did not reach the reducing potential of cocoa in the cell-free systems or cellular model employed. Further, the biological activities examined in colon adenocarcinoma cells did not alter in a stepwise manner that could reflect the gradual changes in composition of bioactive ingredients. In conclusion, the experiments presented here showed that the growing complexity of a mixture of phytochemicals seems to create a new redox bioactive substance rather than enrich the mixture with new activities, characteristic of the compound added. It follows that no simple, predictable relationship can be expected between the chemopreventive potential and the composition of real food items containing a complicated set of non-toxic redox active ingredients. Our observations suggest that the interactions between different bioactive compounds and food matrix components are cooperating factors determining the final bioactivity of foods.
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•Mixtures of cocoa bioactives fail to reach antioxidant power of cocoa.•Mixtures of cocoa antioxidants do not reflect bioactivity of individual compounds.•Interactions between bioactive compounds determine the final bioactivity of foods.•Each mixture composition studied apparently created a new redox active substance.