Single dilution ELISA for detection of serum antibody to foot-and-mouth disease virus in cattle

• Published in: Veterinary microbiology Vol. 18; no. 3-4; p. 273
• Main Authors: Westbury, H A, Chamnanpood, P, Tangchaitrong, S, Doughty, W J
• Format: Journal Article
• Language: English
• Published: Netherlands 01-12-1988
• Subjects: Animals; Antibodies, Viral - analysis; Aphthovirus - immunology; Aphthovirus - isolation & purification; Cattle; Cattle Diseases - diagnosis; Enzyme-Linked Immunosorbent Assay - methods; Foot-and-Mouth Disease - diagnosis; Reference Values; Thailand; Thailand
• ISSN: 0378-1135
• DOI: 10.1016/0378-1135(88)90093-4

A single dilution blocking ELISA was developed and evaluated for measuring serum antibody to foot-and-mouth disease virus (FMDV). Basic parameters of the assay were established and a positive-negative threshold determined from testing 176 specific antibody negative sera from Australian cattle. Sera collected from immunised animals in Thailand were tested by ELISA and virus-neutralisation (VN) tests and the results compared. A positive correlation between ELISA and VN titres was recorded for each of the 3 FMDV serotypes endemic in Thailand, with the overall correlation coefficient being r = 0.8990. A positive correlation for each of the serotypes was also found between ELISA titre and the degree of blocking (percentage inhibition) of each test serum at a dilution of 1:16, with the overall correlation being r = 0.8704. This simplified ELISA was sensitive, specific and gave reproducible results, and had the potential to test quickly and efficiently a considerable number of sera.