Enhancement of 1,4-Dihydroxy-2-Naphthoic Acid Production by Propionibacterium freudenreichii ET-3 Fed-Batch Culture

• Published in: Applied and Environmental Microbiology Vol. 73; no. 10; pp. 3137 - 3143
• Main Authors: Furuichi, Keisuke, Katakura, Yoshio, Ninomiya, Kazuaki, Shioya, Suteaki
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-05-2007
• Subjects: Acids; Anaerobiosis; Bacteria; Bacteriology; Biological and medical sciences; Biotechnology; Carbon - metabolism; Enzyme Inhibitors - pharmacology; Fermentation; Fundamental and applied biological sciences. Psychology; Lactose; Lactose - metabolism; Microbiology; Naphthols - metabolism; Oxygen - metabolism; Physiology and Biotechnology; Propionates - pharmacology; Propionibacterium - metabolism; Propionibacterium freudenreichii; Vitamin K 2 - metabolism; Production; Propionibacteriaceae; Bacteria; Actinomycetes; Propionibacterium freudenreichii; Culture; Semicontinuous
• ISSN: 0099-2240; 1098-5336
• DOI: 10.1128/AEM.01307-06

The production of 1,4-dihydroxy-2-naphthoic acid (DHNA) was investigated using a fed-batch culture of Propionibacterium freudenreichii ET-3. DHNA is a precursor of menaquinone (MK) and is transformed to MK by combination with an isoprenoid unit. We found that ET-3 stopped MK production and increased DHNA production in an anaerobic fed-batch culture by maintaining the lactose concentration at approximately zero. The maximum DHNA concentration observed in the anaerobic fed-batch culture was markedly higher than the maximum DHNA concentration observed in an anaerobic batch culture. Moreover, MK or DHNA production was affected by the lactose feeding rate; this suggests that lactose metabolism participates in the syntheses of these products. On the other hand, accumulation of propionate was found to inhibit DHNA production in the fed-batch culture. Based on the fact that ET-3 increases DHNA production in an aerobic culture by consuming propionate, we carried out a cultivation experiment in which an anaerobic fed-batch culture was switched to an anaerobic batch culture and found that the DHNA production was increased to a greater extent than the DHNA production in an anaerobic fed-batch culture. These results suggest that DHNA production by ET-3 is markedly influenced by carbon source limitation and the oxygen supply.