Detection of target DNA by electrochemical method

• Published in: SENS ACTUATORS, B CHEM Vol. 76; no. 1; pp. 215 - 219
• Main Authors: Maruyama, K, Motonaka, J, Mishima, Y, Matsuzaki, Y, Nakabayashi, I, Nakabayashi, Y
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Elsevier B.V 01-06-2001
• Subjects: DNA; Electrochemical detection; Interfaces (materials); osmium; Osmium complex; phenanthroline; sensors; Square wave voltammetry; Square wave voltammetry; Electrochemical detection; DNA; Osmium complex
• ISSN: 0925-4005; 1873-3077
• DOI: 10.1016/S0925-4005(01)00645-1

The interaction of the osmium complexes {[Os(bpy)
3]
3+ (bpy=bipyridine), [Os(phen)
3]
2+ (phen=1,10-phenanthroline), [Os(5,6-dmphen)
3]
2+ (5,6-dmphen=5,6-dimethyl-1,10-phenanthroline), and [Os(4,7-dmphen)
3]
2+ (4,7-dmphen=4,7-dimethyl-1,10-phenanthroline)} with calf thymus DNA were studied electrochemically. The binding constant of [Os(5,6-dmphen)
3]
2+,
K=4.2×10
5
M
−1 was found to be larger than those observed for the known metal complex indicator and osmium complexes with methyl phenanthroline ligands. This paper shows that a DNA sensor system with [Os(5,6-dmphen)
3]
2+ as a hybridization indicator and square wave voltammetry, could selectively detect DNA in a range of 6.9×10
−10 to 6.9×10
−5
g/ml.