Nucleotide and deduced amino acid sequence of Mycobacterium leprae manganese superoxide dismutase

• Published in: Nucleic acids research Vol. 17; no. 20; p. 8378
• Main Authors: THANGARAJ, H. S, LAMB, F. I, DAVIS, E. O, COLSTON, M. J
• Format: Journal Article
• Language: English
• Published: Oxford Oxford University Press 25-10-1989
• Subjects: Amino Acid Sequence; Base Sequence; Biological and medical sciences; DNA, Bacterial - genetics; Fundamental and applied biological sciences. Psychology; Gene Library; Genes; Genes, Bacterial; Genes. Genome; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mycobacterium leprae; Mycobacterium leprae - enzymology; Mycobacterium leprae - genetics; superoxide dismutase; Superoxide Dismutase - genetics; Mycobacterium leprae; Complementary DNA; Nucleotide sequence; Mycobacteriales; Enzyme; Mycobacteriaceae; Bacteria; Superoxide dismutase; Actinomycetes; Molecular cloning; Aminoacid sequence; Manganese
• ISSN: 0305-1048; 1362-4962
• DOI: 10.1093/nar/17.20.8378

In order to identify protein coding sequences on M. leprae DNA we obtained a clone (Y3164), isolated from an M. leprae DNA library cloned in lambda gt11 that expresses M. laprae epitopes recognised by monoclonal antibodies raised against the organism. The 2.8 kb insert of Y3164 was used to probe an M. leprae cosmid library. A 3.5 kb Pst1 fragment that hybridised to the Y3164 insert was sequenced. The deduced amino acid sequence from one of the open reading frames (621 bps) exhibits significant homology with known Mn/Fe superoxide dismutase sequences (EC.1.15.1.1).