The clinical utility of a near patient care rapid microarray-based diagnostic test for influenza and respiratory syncytial virus infections in the pediatric setting

• Published in: Diagnostic microbiology and infectious disease Vol. 78; no. 4; pp. 363 - 367
• Main Authors: Nakao, Akihiro, Hisata, Ken, Matsunaga, Nobuaki, Fujimori, Makoto, Yoshikawa, Naomi, Komatsu, Mitsutaka, Kikuchi, Ken, Takahashi, Hiroshi, Shimizu, Toshiaki
• Format: Journal Article
• Language: English
• Published: United States Elsevier Inc 01-04-2014
• Subjects: Adolescent; Child; Child, Preschool; Diagnostic Tests, Routine - methods; Female; Humans; Immunochromatography - methods; Infant; Infectious Disease; Influenza; Influenza, Human - diagnosis; Internal Medicine; Male; Microarray Analysis - methods; Microarray: Immunochromatography; Molecular Diagnostic Techniques - methods; Near patient care test; Orthomyxoviridae - isolation & purification; Pediatrics; Point-of-Care Systems; Rapid antigen test; Respiratory syncytial virus; Respiratory Syncytial Virus Infections - diagnosis; Respiratory Syncytial Virus, Human - isolation & purification; Sensitivity and Specificity; Pediatrics; Near patient care test; Respiratory syncytial virus; Rapid antigen test; Influenza; Microarray: Immunochromatography
• ISSN: 0732-8893; 1879-0070
• DOI: 10.1016/j.diagmicrobio.2013.11.005

Abstract We evaluated the potential clinical utility of an automated near patient molecular assay Verigene Respiratory Virus Plus (RV+) and rapid immunochromatographic antigen tests (RIAT) in the pediatric setting for diagnosis of influenza and respiratory syncytial virus infections when testing was performed by the pediatrician seeing the patient. Overall, with respect to influenza virus, sensitivity and specificity for RIAT were 70.8% and 100%, respectively, compared to 100% and 96.2%, respectively, for RV+. For respiratory syncytial virus, sensitivity and specificity for RIAT were 78.9% and 100%, respectively, compared to 100% and 100%, respectively, for RV+. When RIAT and RV+ sensitivity for influenza virus was compared based on the time the patient presented after onset of fever, the sensitivity of RIAT at 6 hours was 37.5% compared to 100% for RV+. At 12 hours, RIAT improved to 60.9%. This study confirms the clinical utility of RV+ in the pediatric setting.