Bi-stage control of dissolved oxygen to enhance cyclic adenosine monophosphate production by Arthrobacter A302

• Published in: Bioprocess and biosystems engineering Vol. 35; no. 8; pp. 1281 - 1286
• Main Authors: Li, Lei, Chen, Xiaochun, Cheng, Jian, Zhang, Chunwei, Bai, Jianxin, Chen, Yong, Niu, Huanqin, Ying, Hanjie
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer-Verlag 01-10-2012; Springer; Springer Nature B.V
• Subjects: Arthrobacter; Arthrobacter - growth & development; Arthrobacter - metabolism; Bioengineering; Biological and medical sciences; Biotechnology; Cellular biology; Chemistry; Chemistry and Materials Science; Cyclic AMP - biosynthesis; Dissolved oxygen; Environmental Engineering/Biotechnology; Fermentation; Food Science; Fundamental and applied biological sciences. Psychology; Gram-positive bacteria; Industrial and Production Engineering; Industrial Chemistry/Chemical Engineering; Kinetics; Original Paper; Oxygen; Oxygen - metabolism; Oxygen Consumption; A302; Dissolved oxygen; Two-stage oxygen supply control strategy; Cyclic adenosine monophosphate; Adenosine; Production; Bacteria; Actinomycetes; Arthrobacter; Arthrobacter A302
• ISSN: 1615-7591; 1615-7605
• DOI: 10.1007/s00449-012-0715-3

Experiments confirmed dissolved oxygen (DO) definitely affects cyclic adenosine monophosphate (cAMP) production by
Arthrobacter
A302. Production of cAMP by batch fermentation was investigated under various DO conditions. A two-stage DO control strategy was proposed to achieve optimal production of cAMP based on the kinetic analysis: the DO level was controlled at 40% during the first 18 h and then switched to 30%. Relatively high cAMP production (9.9 g L
−1
) was achieved by applying this strategy. The cAMP productivity (0.14 g L
−1
 h
−1
) was also successfully improved by 85.1, 59.3, 15.1 and 28.0%, compared to cases in which DO was uncontrolled or DO levels were held at 20, 30 and 40%, respectively. This is the first report of the use of a two-stage DO control strategy in cAMP production, and it was verified to be an effective method for enhancing the cAMP yield via this strain.