Stable transformation and regeneration of transgenic plants of Pinus radiata D. Don

A biolistic particle delivery system was used to genetically transform embryogenic tissue of Pinus radiata. The introduced DNA contained a uidA reporter gene under the control of either the tandem CaMV 35S or the artificial Emu promoter, and the npt II selectable marker controlled by the CaMV 35S pr...

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Bibliographic Details
Published in:	Plant cell reports Vol. 17; no. 6/7; pp. 460 - 468
Main Authors:	Walter, C, Grace, L.J, Wagner, A, White, D.W.R, Walden, A.R, Donaldson, S.S, Hinton, H, Gardner, R.C, Smith, D.R
Format:	Journal Article
Language:	English
Published:	Berlin Springer 01-04-1998 Springer Nature B.V
Subjects:	Bacteria biolistics Biological and medical sciences Biotechnology clones developmental stages DNA embryo (plant) Fundamental and applied biological sciences. Psychology gene expression gene transfer Genetic engineering Genetic technics genetic transformation genetic variation genetic vectors Genomes histochemistry Integration localization Methods. Procedures. Technologies micropropagation nptii genes Pine trees Pinus radiata Polymerase chain reaction promoter regions Promoters regenerative ability Reporter gene reporter genes Somatic embryos Studies Transformation Transgenic animals and transgenic plants Transgenic plants Trees uida reporter genes Microprojectile bombardment Embryo Vegetals β-Glucuronidase Genetic transformation Enzyme Transferases Tissue culture Pinus radiata Gene expression Kanamycin kinase Regeneration Reporter gene Glycosidases Gymnospermae Softwood forest tree Hydrolases Coniferales Spermatophyta Transgenic plant O-Glycosidases Transformation Biolistic Embryogenic tissue Key wordsPinus radiata
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