A noninvasive, direct real-time PCR method for sex determination in multiple avian species

Polymerase chain reaction (PCR)‐based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not con...

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Bibliographic Details
Published in:	Molecular ecology resources Vol. 11; no. 2; pp. 415 - 417
Main Authors:	BRUBAKER, JESSICA L., KAROUNA-RENIER, NATALIE K., CHEN, YU, JENKO, KATHRYN, SPRAGUE, DANIEL T., HENRY, PAULA F. P.
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Publishing Ltd 01-03-2011 Wiley Subscription Services, Inc
Subjects:	Animals birds Birds - genetics Female Male molecular sexing noninvasive sampling Polymerase Chain Reaction - methods real-time PCR Sex Determination Analysis - methods Test methods
Online Access:	Get full text
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Summary:	Polymerase chain reaction (PCR)‐based methods to determine the sex of birds are well established and have seen few modifications since they were first introduced in the 1990s. Although these methods allowed for sex determination in species that were previously difficult to analyse, they were not conducive to high‐throughput analysis because of the laboriousness of DNA extraction and gel electrophoresis. We developed a high‐throughput real‐time PCR‐based method for analysis of sex in birds, which uses noninvasive sample collection and avoids DNA extraction and gel electrophoresis.
Bibliography:	istex:4E1F1163D191370DF64FD80A5D9ED70237A8564C ArticleID:MEN2951 ark:/67375/WNG-GZRPNS92-N These authors contributed equally to this manuscript. ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23
ISSN:	1755-098X 1755-0998
DOI:	10.1111/j.1755-0998.2010.02951.x