Sphingomonas cloacae sp. nov., a nonylphenol-degrading bacterium isolated from wastewater of a sewage-treatment plant in Tokyo

• Published in: International journal of systematic and evolutionary microbiology Vol. 51; no. 2; pp. 603 - 610
• Main Authors: Fujii, K, Urano, N, Ushio, H, Satomi, M, Kimura, S
• Format: Journal Article
• Language: English
• Published: Reading Soc General Microbiol 01-03-2001; Society for General Microbiology
• Subjects: Animal, plant and microbial ecology; Bacterial Typing Techniques; Bacteriology; Biodegradation, Environmental; Biological and medical sciences; DNA, Ribosomal - genetics; Environmental Microbiology; Fatty Acids - analysis; Fundamental and applied biological sciences. Psychology; Japan, Tokyo; Microbial ecology; Microbiology; Microflora of plants; Molecular Sequence Data; Phenols - metabolism; polyphenols; RNA, Ribosomal, 16S - genetics; rrNA 16S gene; Sewage - microbiology; Sphingomonas - classification; Sphingomonas - metabolism; Sphingomonas cloacae; Systematics; Terminology as Topic; Asia; Honshu; Japan; Ribosomal DNA; Lipids; Sphingomonas; Phylogeny; Sewage treatment plant; Waste water; Plant; Treatment; Content; 16S-RNA; Bacteria; Isolate; Isolation; Waste water purification
• ISSN: 1466-5026; 1466-5034
• DOI: 10.1099/00207713-51-2-603

K Fujii, N Urano, H Ushio, M Satomi and S Kimura
Laboratory of Marine Biochemistry, Tokyo University of Fisheries, Konan, Minato, Tokyo 108-8477, Japan
A nonylphenol-assimilating bacterium isolated at a sewage-treatment plant
in Tokyo was studied phenotypically, genotypically and phylogenetically.
Analysis of the 16S rDNA sequence, the G+C content of the DNA (63 mol%) and
the isoprenoid quinone composition, as well as the presence of
sphingoglycolipid and the whole-cell fatty acid profile, revealed that the
isolate is a member of the genus Sphingomonas. However, the sequence
similarity of the 16S rDNA with that of known Sphingomonas spp. was found
to be at most 96%, implying that the isolate is distinctive. Furthermore,
the results of DNA--DNA hybridization experiments and its physiological
characteristics clearly indicated that the isolate represents a new
Sphingomonas species, for which the name Sphingomonas cloacae is proposed;
strain S-3(T) (=JCM 10874(T)=IAM 14885(T)) is the type strain.