A single amino acid substitution in the 126-kDa protein of pepper mild mottle virus controls replication and systemic movement into upper non-inoculated leaves of bell pepper plants

Previously, we generated attenuated variants of pepper mild mottle virus by replacing residue 649 in the 126-kDa replicase protein with various amino acids. Here, we examined the biological properties of the 16 variants that caused either mild mosaic or no mosaic. All but one (A649N) of the mild-mos...

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Bibliographic Details
Published in:	Archives of virology Vol. 156; no. 5; pp. 897 - 901
Main Authors:	Nakazono-Nagaoka, E., Omura, T., Uehara-Ichiki, T.
Format:	Journal Article
Language:	English
Published:	Vienna Springer Vienna 01-05-2011 Springer Springer Nature B.V
Subjects:	Amino Acid Substitution Amino acids Antigens Biological and medical sciences Biomedical and Life Sciences Biomedicine Brief Report Capsicum - virology Cloning Fundamental and applied biological sciences. Psychology Infectious Diseases Leaves Medical Microbiology Microbiology Miscellaneous Mutant Proteins - genetics Mutant Proteins - metabolism Phytopathology. Animal pests. Plant and forest protection Plant Leaves - virology Plant viruses and viroids Proteins Tobamovirus - pathogenicity Viral Proteins - genetics Viral Proteins - metabolism Virology Virulence Virus Replication Viruses Japan Coat Protein Successive Inoculation Mild Mosaic Wild Strain Pepper Plant Virus Plant pathogen Vegetable crop Dicotyledones Angiospermae Flavoring crop Tobamovirus Capsicum annuum Replication Spermatophyta Solanaceae Protein
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Summary:	Previously, we generated attenuated variants of pepper mild mottle virus by replacing residue 649 in the 126-kDa replicase protein with various amino acids. Here, we examined the biological properties of the 16 variants that caused either mild mosaic or no mosaic. All but one (A649N) of the mild-mosaic-inducing strains replicated at higher levels in pepper plants and systemically moved at higher rates into the upper non-inoculated leaves than the no-mosaic strains. C1421, previously selected for practical use, not only caused mild symptoms but also had an especially high replication rate in pepper plants and spread more efficiently into the upper non-inoculated leaves.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2
ISSN:	0304-8608 1432-8798
DOI:	10.1007/s00705-011-0919-x