Western blot assay for the detection of antibodies to bovine immunodeficiency-like virus in experimentally inoculated cattle, sheep, and goats

• Published in: Archives of virology Vol. 116; no. 1/4; pp. 119 - 131
• Main Authors: Whetstone, C.A, VanDerMaaten, M.J, Miller, J.M
• Format: Journal Article
• Language: English
• Published: Wien Springer 01-01-1991; New York, NY
• Subjects: AIDS/HIV; Animals; antibodies; Antibodies, Viral - analysis; Antibodies, Viral - immunology; antibody formation; Antigens, Viral - immunology; Biological and medical sciences; Blotting, Western - methods; bovine immunodeficiency virus; Cattle; Cells, Cultured; experimental infection; Fundamental and applied biological sciences. Psychology; Goats; Immunization; immunoblotting; Immunodeficiency Virus, Bovine - growth & development; Immunodeficiency Virus, Bovine - immunology; Immunodiffusion; Lentivirus; Lentivirus Infections - immunology; Lung - microbiology; Microbiology; serum; Sheep; Techniques used in virology; viral antigens; Viral Proteins - immunology; Virology; Virus; Antigen; Proteins; Experimental disease; Immune response; Antibody; Animal; Retroviridae; Immunoblotting assay; Lentivirinae; Detection; Humoral immunity
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/bf01319236

A cocultivation method was used to establish a cytocidal bovine immunodeficiency-like virus (BIV) infection in primary fetal bovine lung (FBL) cell cultures. Cultures were monitored for virus production using radial immunodiffusion and agar gel immunodiffusion. Pelleted virus and detergent (CHAPS)-solubilized infected cell lysates from BIV-infected cell cultures were compared as sources of antigen for Western blots. Pelleted virus preparations from FBL-BIV cell cultures produced the best antigen for Western blot. Sheep and goats were inoculated with BIV and serum antibody responses were monitored up to 1 year post inoculation (PI). Sera from experimentally infected cattle, sheep, and goats reacted in Western blot assay with BIV viral induced polypeptides gp110, p72, p55, p50, gp42, p38, p26, p24, p18, p15, and p13. Antibodies to p26 were detected as early as 2 weeks PI in cattle, sheep, and goats. Antibodies to gp110 were detected by 4 to 6 weeks PI in cattle, and by 9 months PI in sheep and goats. Antibodies to BIV proteins were still evident in cattle sera 2 1/2 years PI, and in sheep and goat sera 1 year Pl.