Expression of the cell adhesion molecules on leukocytes that demarginate during acute maximal exercise

• Published in: Journal of applied physiology (1985) Vol. 86; no. 3; pp. 970 - 976
• Main Authors: van Eeden, Stephan F, Granton, John, Hards, Jennifer M, Moore, Barbara, Hogg, James C
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Am Physiological Soc 01-03-1999; American Physiological Society
• Subjects: Adult; Biological and medical sciences; Blood; Catecholamines - metabolism; CD18 Antigens - biosynthesis; Cell Adhesion Molecules - biosynthesis; Cellular biology; Exercise; Exercise - physiology; Exercise Test; Female; Fundamental and applied biological sciences. Psychology; Fundamental immunology; Humans; Immunobiology; L-Selectin - biosynthesis; Leukocyte Count; Leukocytes; Leukocytes - enzymology; Leukocytes - metabolism; Leukocytes - physiology; Macrophage-1 Antigen - biosynthesis; Male; Middle Aged; Modulation of the immune response (stimulation, suppression); Molecules; Neutrophils - enzymology; Neutrophils - metabolism; Peroxidase - metabolism; Physical exercise; Human; Integrin; Immunomodulation; Leukocyte; Pulmonary sequestration; Bicycle ergometer; Cell adhesion molecule; Pulmonary circulation; Neutrophil
• ISSN: 8750-7587; 1522-1601
• DOI: 10.1152/jappl.1999.86.3.970

Pulmonary Research Laboratory, St. Paul's Hospital, University of
British Columbia, Vancouver, British Columbia, Canada V6Z 1Y6
The pulmonary vascular bed is an important
reservoir for the marginated pool of leukocytes that can be mobilized
by exercise or catecholamines. This study was designed to determine the
phenotypic characteristics of leukocytes that are mobilized into the
circulation during exercise. Twenty healthy volunteers performed
incremental exercise to exhaustion [maximal
O 2 consumption
( O 2 max )]
on a cycle ergometer. Blood was collected at baseline, at 3-min
intervals during exercise, at
O 2 max , and 30 min
after exercise. Total white cell, polymorphonuclear leukocyte (PMN),
and lymphocyte counts increased with exercise to
O 2 max
( P  < 0.05). Flow cytometric analysis
showed that the mean fluorescence intensity of L-selectin on PMN (from
14.9 ± 1 at baseline to 9.5 ± 1.6 at
O 2 max ,
P   < 0.05) and lymphocytes (from 11.7 ± 1.2 at baseline to 8 ± 0.8   at
O 2 max ,
P  < 0.05) decreased with exercise.
Mean fluorescence intensity of CD11b on PMN increased with exercise
(from 10.2 ±   0.6 at baseline to 25 ± 2.5 at
O 2 max ,
P  < 0.002) but remained unchanged on
lymphocytes. Myeloperoxidase levels in PMN did not change with
exercise. In vitro studies showed that neither catecholamines nor
plasma collected at
O 2 max during
exercise changed leukocyte L-selectin or CD11b levels. We conclude that
PMN released from the marginated pool during exercise express low
levels of L-selectin and high levels of CD11b.
neutrophils; cell adhesion molecules; integrins; sequestration